Summary of Study ST000428

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000332. The data can be accessed directly via it's Project DOI: 10.21228/M84G7M This work is supported by NIH grant, U2C- DK119886.

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Study IDST000428
Study TitleTargeted metabolomics of gastrocnemius tissue samples obtained from 20 month old (old) mice- Both Sham and after inducing lung injury (part II)
Study TypeTargeted metabolomic analysis
Study SummaryIntroduction: Older patients are more likely to acquire and die from acute respiratory distress syndrome (ARDS) and muscle weakness may be more significant in older survivors. Recent data implicate muscle ring finger protein 1 (MuRF1) in lung injury-induced skeletal muscle atrophy in young mice and identify an alternative role for MuRF1 in cardiac metabolism regulation through inhibition of fatty acid oxidation. Objectives: To develop a model of lung injury-induced muscle wasting in old mice and to evaluate the skeletal muscle metabolomic profile of adult and old acute lung injury (ALI) mice. Methods: Young (2 month), adult (6 month) and old (20 month) male C57Bl6J mice underwent Sham (intratracheal H2O) or ALI [intratracheal E. coli lipopolysaccharide (i.t. LPS)] conditions and muscle functional testing. Metabolomic analysis on gastrocnemius muscle was performed using gas chromatography-mass spectrometry (GC-MS). Results: Old ALI mice had increased mortality and failed to recover skeletal muscle function compared to adult ALI mice. Muscle MuRF1 expression was increased in old ALI mice at day 3. Non-targeted muscle metabolomics revealed alterations in amino acid biosynthesis and fatty acid metabolism in old ALI mice. Targeted metabolomics of fatty acid intermediates (acyl-carnitines) and amino acids revealed a reduction in long chain acyl-carnitines in old ALI mice. Conclusion: This study demonstrates age-associated susceptibility to ALI-induced muscle wasting which parallels a metabolomic profile suggestive of altered muscle fatty acid metabolism. MuRF1 activation may contribute to both atrophy and impaired fatty acid oxidation, which may synergistically impair muscle function in old ALI mice.
Institute
University of North Carolina;Duke University
DepartmentUNC McAllister Heart Institute;Duke Molecular Physiology Institute
LaboratoryMultiple Centers
Last NameIlaiwy;WIllis
First NameAmro;Monte
Address111 Mason Farm road, Chapel Hill, North Carolina, 27599-7126, USA
Emailmonte_willis@med.unc.edu, amroilaiwy@gmail.com
Phone210-596-0171
Submit Date2016-07-05
Analysis Type DetailGC-MS
Release Date2016-09-23
Release Version1
Amro Ilaiwy Amro Ilaiwy
Monte WIllis Monte WIllis
https://dx.doi.org/10.21228/M84G7M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample
SA021634A110ALI 20mth
SA021635A109ALI 20mth
SA021636A108ALI 20mth
SA021637A111ALI 20mth
SA021638A46ALI 20mth
SA021639A48ALI 20mth
SA021640A47ALI 20mth
SA021641A106ALI 20mth
SA021642A45ALI 20mth
SA021643A05ALI 20mth
SA021644A12Sham 20mth
SA021645A11Sham 20mth
SA021646A10Sham 20mth
SA021647A09Sham 20mth
SA021648A32Sham 20mth
SA021649A33Sham 20mth
SA021650A52Sham 20mth
SA021651A50Sham 20mth
SA021652A34Sham 20mth
SA021653A08Sham 20mth
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