Summary of Study ST000469
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000360. The data can be accessed directly via it's Project DOI: 10.21228/M8HK59 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000469 |
| Study Title | Enterococcus faecalis nucleotide profiles - pilot |
| Study Type | Comparison between wild-type and mutant strain, as well as providing varying cell count input for WT |
| Study Summary | E. faecalis OG1RF and rel/relQ will be grown in FMC-AUG to OD600 = 0.25. 3E10 cells will be harvested by fast filtration, scraped off of membrane into ice-cold PBS, and pelleted by centrifugation. This process will be repeated with OG1RF only to harvest 3E9 and 3E8 cells. A second OG1RF sample of 3E10 cells will be scraped into formic acid; only acid-soluble extract willl be sent for this sample. |
| Institute | University of Florida |
| Department | SECIM |
| Laboratory | Lemos-Abranches |
| Last Name | Kajfasz |
| First Name | Jessica |
| Address | 1395 Center Drive, PO Box 100424 Gainesville, FL 32610 |
| jkajfasz@dental.ufl.edu | |
| Phone | 352-273-6675 |
| Submit Date | 2016-09-10 |
| Num Groups | 5 |
| Total Subjects | 5 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-10-03 |
| Release Version | 1 |
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Factors:
Subject type: Bacterial cells; Subject species: Enterococcus faecalis (Factor headings shown in green)
| mb_sample_id | local_sample_id | Strain | Resuspension |
|---|---|---|---|
| SA023663 | rel/relQ_3E10 | (p)ppGpp0 | PBS |
| SA023664 | OG1RF_3E10_acid | WT | 1M Formic Acid |
| SA023665 | OG1RF_3E8 | WT | PBS |
| SA023666 | OG1RF_3E9 | WT | PBS |
| SA023667 | OG1RF_3E10 | WT | PBS |
| Showing results 1 to 5 of 5 |
