Summary of Study ST000484

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000367. The data can be accessed directly via it's Project DOI: 10.21228/M8MC7X This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files
Study IDST000484
Study TitleAmino Acid Quantifcation of obese patients on a 16 week caloric restriction from muscle biopsy
Study Typetimecourse, quantitative measurements of amino acid
Study SummaryCaloric restriction (CR) improves insulin sensitivity and reduces the incidence of diabetes in obese individuals. The underlying mechanisms whereby CR improves insulin sensitivity are not clear. We evaluated the effect of 16 weeks of CR on whole-body insulin sensitivity by pancreatic clamp before and after CR in 11 obese participants (BMI = 35 kg/m2) compared with 9 matched control subjects (BMI = 34 kg/m2). Compared with the control subjects, CR increased the glucose infusion rate needed to maintain euglycemia during hyperinsulinemia, indicating enhancement of peripheral insulin sensitivity. This improvement in insulin sensitivity was not accompanied by changes in skeletal muscle mitochondrial oxidative capacity or oxidant emissions, nor were there changes in skeletal muscle ceramide, diacylglycerol, or amino acid metabolite levels. However, CR lowered insulin-stimulated thioredoxin-interacting protein (TXNIP) levels and enhanced nonoxidative glucose disposal. These results support a role for TXNIP in mediating the improvement in peripheral insulin sensitivity after CR.
Institute
Mayo Clinic
DepartmentEndocrinology
LaboratoryMayo Clinic Metabolomics Resource Core
Last NameNair
First NameSreekumaran
Address200 First Street SW, Rochester, MN 55905
EmailNair.K@mayo.edu
Phone507-285-2415
Submit Date2016-09-23
PublicationsMechanism by Which Caloric Restriction Improves Insulin Sensitivity in Sedentary Obese Adults. DOI: 10.2337/db15-0675
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2017-11-20
Release Version1
Sreekumaran Nair Sreekumaran Nair
https://dx.doi.org/10.21228/M8MC7X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Factors:

Subject type: Animal; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id time (mins) Category
SA024622ms5370-111 Caloric Restriction
SA024623ms5370-331 Caloric Restriction
SA024624ms5370-311 Caloric Restriction
SA024625ms5370-231 Caloric Restriction
SA024626ms5370-11 Caloric Restriction
SA024627ms5370-191 Caloric Restriction
SA024628ms5370-131 Caloric Restriction
SA024629ms5370-91 Caloric Restriction
SA024630ms5370-51 Caloric Restriction
SA024631ms5370-31 Caloric Restriction
SA024632ms5370-71 Caloric Restriction
SA024633ms5370-391 Control
SA024634ms5370-251 Control
SA024635ms5370-291 Control
SA024636ms5370-371 Control
SA024637ms5370-351 Control
SA024638ms5370-271 Control
SA024639ms5370-211 Control
SA024640ms5370-171 Control
SA024641ms5370-151 Control
SA024642ms5370-122 Caloric Restriction
SA024643ms5370-202 Caloric Restriction
SA024644ms5370-322 Caloric Restriction
SA024645ms5370-102 Caloric Restriction
SA024646ms5370-82 Caloric Restriction
SA024647ms5370-342 Caloric Restriction
SA024648ms5370-142 Caloric Restriction
SA024649ms5370-62 Caloric Restriction
SA024650ms5370-242 Caloric Restriction
SA024651ms5370-22 Caloric Restriction
SA024652ms5370-42 Caloric Restriction
SA024653ms5370-382 Control
SA024654ms5370-362 Control
SA024655ms5370-402 Control
SA024656ms5370-302 Control
SA024657ms5370-182 Control
SA024658ms5370-222 Control
SA024659ms5370-162 Control
SA024660ms5370-262 Control
SA024661ms5370-282 Control
Showing results 1 to 40 of 40
  logo