Summary of Study ST000678
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000480. The data can be accessed directly via it's Project DOI: 10.21228/M8102Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000678 |
| Study Title | Functional genomics study on Non Alcoholic fatty liver disease (NAFLD) |
| Study Type | MS analysis |
| Study Summary | In this experiment, vector , GCKR (WT), GCKR(P446L), PPP1R3B, TM6SF2 (WT and E167K) overexpression stable HuH-7_Lok cell lines as well as Non-target, GCKR shRNA (52621), PPP1R3B shRNA (2640), TM6SF2 shRNA (382426) knockdown HuH-7_Lok cell lines are used to do the lipidomics experiments 3 X 100 mm dishes of cells were seeded (1x106 cells/dish) for each cell line and incubated at 37? C for 24 hours, then replaced medium with delipidated serum DMEM medium. After 24 hours, treated with BSA-OA (200?M) for another 24 hours before the collection of cells Add 4mL/dish 50 mM ammonium acetate wash cells and then quench cells by adding liquid nitrogen covering all the surface of dish. Store the cell samples in -80?C freezer. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-05 |
| Num Groups | 6 |
| Total Subjects | 1 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
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Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Construct |
|---|---|---|
| SA038944 | S00026025 | GCKR(P446L) |
| SA038945 | S00026024 | GCKR(P446L) |
| SA038946 | S00026023 | GCKR(P446L) |
| SA038947 | S00026021 | GCKR(WT) |
| SA038948 | S00026020 | GCKR(WT) |
| SA038949 | S00026022 | GCKR(WT) |
| SA038950 | S00026026 | NT |
| SA038951 | S00026027 | NT |
| SA038952 | S00026028 | NT |
| SA038953 | S00026029 | PPP1R3B_2640 |
| SA038954 | S00026030 | PPP1R3B_2640 |
| SA038955 | S00026031 | PPP1R3B_2640 |
| SA038956 | S00026017 | PPP1R3B_3 |
| SA038957 | S00026018 | PPP1R3B_3 |
| SA038958 | S00026019 | PPP1R3B_3 |
| SA038959 | S00026015 | Vector |
| SA038960 | S00026016 | Vector |
| SA038961 | S00026014 | Vector |
| Showing results 1 to 18 of 18 |
