Summary of Study ST000685
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000484. The data can be accessed directly via it's Project DOI: 10.21228/M8H03P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000685 |
| Study Title | Lipidomics analysis for human liver cell lines |
| Study Type | MS analysis |
| Study Summary | On day 1, seed 1 million cells/dish Huh7_Lok cells (vector, GCKR(WT), GCKR(P446L), Non-Target, GCKR KD and PPP1R3B/OE) in 100 mm dishes with DMEM complete medium and incubate at 37C for 24 h. On day 2, replace DMEM medium with delipidated serum medium for these cells and incubate for another 24 h. On day 3, add heated Oleic Acid-Albumin into each dish at final concentration of 200?M for 24h. On day 4, collect cells with Trypsin/EDTA lysis and count cells with automated cell counter and calculate total cell numbers in each sample, then centrifuge cells down and take out the supernatant and keep cells in -80 C freezer. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-05 |
| Num Groups | 6 |
| Total Subjects | 1 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-04-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Construct |
|---|---|---|
| SA039079 | S00027766 | GCKR_52621 |
| SA039080 | S00027767 | GCKR_52621 |
| SA039081 | S00027768 | GCKR_52621 |
| SA039073 | S00027759 | GCKR(P446L) |
| SA039074 | S00027758 | GCKR(P446L) |
| SA039075 | S00027760 | GCKR(P446L) |
| SA039076 | S00027756 | GCKR(WT) |
| SA039077 | S00027755 | GCKR(WT) |
| SA039078 | S00027757 | GCKR(WT) |
| SA039082 | S00027764 | NT |
| SA039083 | S00027763 | NT |
| SA039084 | S00027765 | NT |
| SA039085 | S00027762 | PPP1R3B |
| SA039086 | S00027761 | PPP1R3B |
| SA039087 | S00027753 | Vector |
| SA039088 | S00027752 | Vector |
| SA039089 | S00027754 | Vector |
| Showing results 1 to 17 of 17 |
