Summary of Study ST000819
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000557. The data can be accessed directly via it's Project DOI: 10.21228/M8P39J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000819 |
| Study Title | Looking for changes in lipid levels with knock out of ACADM (shACADM) (part II) |
| Study Type | MS analysis |
| Study Summary | GSC 8-11 cells (20 million) were transduced with lentivirus coding for shACADM 4, 6, or scr (control). ACADM is an enzyme responsible for the metabolism of medium chain fatty acids (C4-C12). A round of selection was performed using puromycin to ensure only cells that had been transduced survived. Knockdown of ACADM was confirmed via western blot. Pellets were collected by centrifuging cells for 10 min at max speed. The pellets were washed and each sample was split into quadruplicate. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-07-14 |
| Num Groups | 3 |
| Total Subjects | 3 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-02-07 |
| Release Version | 1 |
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Factors:
Subject type: HUMAN; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Transduced with |
|---|---|---|
| SA045798 | S00021734 | shACADM4 |
| SA045799 | S00021733 | shACADM4 |
| SA045800 | S00021731 | shACADM4 |
| SA045801 | S00021732 | shACADM4 |
| SA045802 | S00021738 | shACADM6 |
| SA045803 | S00021737 | shACADM6 |
| SA045804 | S00021736 | shACADM6 |
| SA045805 | S00021735 | shACADM6 |
| SA045806 | S00021742 | shACADMscr |
| SA045807 | S00021740 | shACADMscr |
| SA045808 | S00021739 | shACADMscr |
| SA045809 | S00021741 | shACADMscr |
| Showing results 1 to 12 of 12 |
