Summary of Study ST001026

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000684. The data can be accessed directly via it's Project DOI: 10.21228/M8868G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)
Study IDST001026
Study TitleTCA cycle metabolomics of H3K27M Cell Nucleus Fraction and Cell Mitonchonrdial Fraction (Part-IV)
Study SummaryTesting TCA concentrations of Diffuse Intrinsic Pontine Gliomas (DIPG) cellines with H3K27M mutations. Preliminary studies show H3K27M tumor cells are addicted to Gln for survival. Removal of Gln from media resulted in tumor cell death which was rescued by the addition of α-KG. These data show that Gln is taken up and metabolized by H3K27M tumor cells and that Gln derived α-KG is critical for the survival of these tumors. Interestingly, tumor cell death with Gln deprivation was similar to the effect of the JMJD3 inhibitor GSKJ4. Therefore, Gln derived α-KG may be required for both anaplerosis and to drive JMJD3 demethylation. We hypothesize that H3K27M tumors are reliant on α-KG that is derived from Gln to drive the TCA cycle and further decrease H3K27 methylation levels. Furthermore, inhibition of Gln metabolism may represent a novel therapeutic approach for tumors with this mutation. In this study, TCA cycle metabolomics are analyzed of H3K27M cells grown in regular glutamine media, glutamine free media, and glutamine free media with alpha-ketoglutarate. Additionally, cell nucleus and cell mitochrondial fractions are run separately.
Institute
Mayo Clinic
Last NameDaniels
First NameDavid
Address200 First Street SW Rochester, MN 55905
Emaildaniels.david@mayo.edu
Phone507-284-2511
Submit Date2018-07-18
Analysis Type DetailGC-MS
Release Date2020-07-15
Release Version1
David Daniels David Daniels
https://dx.doi.org/10.21228/M8868G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id sample type Grouping
SA064476ms6808 - 18Mito Fraction IV aKG
SA064477ms6808 - 17Mito Fraction IV aKG
SA064478ms6808 - 16Mito Fraction IV aKG
SA064470ms6808 - 10Mito Fraction IV GF
SA064471ms6808 - 12Mito Fraction IV GF
SA064472ms6808 - 11Mito Fraction IV GF
SA064473ms6808 - 6Mito Fraction IV REG
SA064474ms6808 - 5Mito Fraction IV REG
SA064475ms6808 - 4Mito Fraction IV REG
SA064485ms6808 - 35Mito Fraction XVII aKG
SA064486ms6808 - 36Mito Fraction XVII aKG
SA064487ms6808 - 34Mito Fraction XVII aKG
SA064479ms6808 - 28Mito Fraction XVII GF
SA064480ms6808 - 30Mito Fraction XVII GF
SA064481ms6808 - 29Mito Fraction XVII GF
SA064482ms6808 - 24Mito Fraction XVII REG
SA064483ms6808 - 22Mito Fraction XVII REG
SA064484ms6808 - 23Mito Fraction XVII REG
SA064494ms6808 - 15Nucleus fraction IV aKG
SA064495ms6808 - 13Nucleus fraction IV aKG
SA064496ms6808 - 14Nucleus fraction IV aKG
SA064488ms6808 - 8Nucleus fraction IV GF
SA064489ms6808 - 7Nucleus fraction IV GF
SA064490ms6808 - 9Nucleus fraction IV GF
SA064491ms6808 - 2Nucleus fraction IV REG
SA064492ms6808 - 3Nucleus fraction IV REG
SA064493ms6808 - 1Nucleus fraction IV REG
SA064503ms6808 - 31Nucleus fraction XVII aKG
SA064504ms6808 - 32Nucleus fraction XVII aKG
SA064505ms6808 - 33Nucleus fraction XVII aKG
SA064497ms6808 - 25Nucleus fraction XVII GF
SA064498ms6808 - 26Nucleus fraction XVII GF
SA064499ms6808 - 27Nucleus fraction XVII GF
SA064500ms6808 - 19Nucleus fraction XVII REG
SA064501ms6808 - 20Nucleus fraction XVII REG
SA064502ms6808 - 21Nucleus fraction XVII REG
Showing results 1 to 36 of 36
  logo