Summary of Study ST001410

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000966. The data can be accessed directly via it's Project DOI: 10.21228/M8TM42 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001410
Study TitlePatterns in metabolite pools show that phytoplankton leave a taxon-specific signature on particulate carbon: Surface samples from the North Pacific Subtropical Gyre to North Pacific Transition Zone
Study TypeMarine metabolomics surface samples from the North Pacific Subtropical Gyre to North Pacific Transition Zone
Study SummaryIn the surface ocean, carbon is fixed by phytoplankton and respired by the entire marine community at an astonishingly high rate. At any point in time, the difference between these two processes yields a carbon pool in surface particles that is a combination of both freshly fixed and partially degraded material. On a molecular level, we have a limited knowledge of the small molecules, or metabolites, within this pool. Specific metabolites have been shown to be responsible for fueling respiration, maintaining organismal interactions, and transferring energy throughout the microbial community. Metabolomics, or the direct observation and quantification of the small molecules that are the result of cellular activity, provides an important lens through which we can begin to assess the standing stocks of small compounds that likely fuel a great deal of heterotrophic activity in the surface ocean. Here we describe community metabolomes of particulate material into the North Pacific Ocean and compare the metabolomes to a variety of phytoplankton grown in the lab. Using both targeted and untargeted metabolomics, we identify metabolites in the particulate carbon pool and explore their latitudinal and phylogenetic distributions. This analysis reveals several compounds that have not been previously recognized as abundant components of the marine organic carbon pool. We found that the community metabolome showed distinct differences between the regimes that likely reflects the phytoplankton community present. The community metabolome in surface waters of the subtropical domain was remarkably consistent even when sampled weeks apart, while the northern regions showed a patchier and less reproducible community metabolome. Some individual compounds showed distinct patterns between oceanographic regimes, including homarine, an abundant molecule that can contribute up to 4% of the total particulate carbon pool in marine surface waters. Glutamic acid and glutamine showed opposite patterns in the oceanographic regimes, suggesting differences in community-level nitrogen assimilation in these different regimes. Overall, this study offers a new perspective into particulate carbon composition in oceanographic research, reveals important carbon pools that may fuel the microbial loop, and suggests an altered community-level nitrogen assimilation capacity over the North Pacific transition zone.
Institute
University of Washington
DepartmentSchool of Oceanography
LaboratoryIngalls Lab
Last NameHeal
First NameKatherine
Address1501 NE Boat Street, Marine Science Building, Room G, Seattle, WA, 98195, USA
Emailkheal@uw.edu
Phone206-616-4658
Submit Date2020-06-25
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2021-03-31
Release Version1
Katherine Heal Katherine Heal
https://dx.doi.org/10.21228/M8TM42
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Other; Subject species: Natural mixed marine microbial community (Factor headings shown in green)

mb_sample_id local_sample_id Depth_m Vol_L
SA115661U7_B15 10
SA115662U8_A15 10
SA115663U8_B15 10
SA115664U7_A15 10
SA115665U6_A15 10
SA115666S9C2_B15 10
SA115667S11C1_A15 10
SA115668U9_B15 10
SA115669S12C1_A15 10
SA115670S14C1_B15 10
SA115671S14C1_C15 10
SA115672S14C1_A15 10
SA115673S13C1_B15 10
SA115674S12C1_B15 10
SA115675S13C1_A15 10
SA115676S9C2_A15 10
SA115677U9_A15 10
SA115678S4C2_B15 10
SA115679S4C2_A15 10
SA115680U6_B15 10.5
SA115681S6C2_A15 10.5
SA115682S6C2_B15 10.5
SA115683S8C1_A15 10.5
SA115684S6C1_C15 11
SA115685S6C1_B15 11
SA115686S9C1_B15 11
SA115687S2C1_C15 11
SA115688S6C1_A15 11
SA115689S2C1_B15 11
SA115690S4C1_B15 11.5
SA115691S4C1_A15 11.5
SA115692S4C1_C15 11.5
SA115693S9C1_A15 11.5
SA115694U4_B15 12
SA115695U4_A15 13.5
SA115696U3_B15 15
SA115697U3_A15 15.5
SA115698S2C3_A15 9.5
SA115699TruePoo_Half2NA NA
SA115700TruePoo_Half3NA NA
SA115701TruePoo_Half1NA NA
SA115702QC_Full3NA NA
SA115703TruePoo_Half4NA NA
SA115704QC_Full4NA NA
SA115705TruePoo_Full4NA NA
SA115706QC_Full2NA NA
SA115707FilterBlk_BNA NA
SA115708TruePoo_Full3NA NA
SA115709TruePoo_Full2NA NA
SA115710TruePoo_Full1NA NA
SA115711April1718AqExtracts_1NA NA
SA115712April21AqExtracts_1NA NA
SA115713April1718AqExtracts_3NA NA
SA115714April1718AqExtracts_2NA NA
SA115715FilterBlk_ANA NA
SA115716April21AqExtracts_2NA NA
SA115717April21AqExtracts_3NA NA
SA115718QC_Half4NA NA
SA115719QC_Half3NA NA
SA115720QC_Half2NA NA
SA115721QC_Half1NA NA
SA115722QC_Full1NA NA
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