Summary of Study ST001449

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000993. The data can be accessed directly via it's Project DOI: 10.21228/M8BH7T This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001449
Study TitleMetabolomics of lung injury after allogeneic hematopoietic cell transplantation - Colon DI-FTMS
Study Typepreliminary data
Study SummaryAllogeneic hematopoietic cell transplantation (allo-HCT) is a potentially curative treatment option for a variety of hematological malignancies. Interactions between the donor immune system and the patient tissue result in a disease, called GVHD. The pathophysiology of acute GVHD can be hypothesized in three sequential phases: cytokine storm and activation of the antigen-presenting cells (APC), donor T cell activation and effector cell phase. Idiopathic pneumonia syndrome (IPS) is one of the most deleterious complications after allogeneic HCT and is considered not only to be related to conditioning regimen toxicity but also represents an end organ damage caused by allo-reactive T cells, therefore making the lung susceptible to a two-pronged attack, one of which overlaps with GVHD causing other target organ injury. IPS results in mortality of up to 90% of patients. We will use a murine model of IPS and GVHD which is well established in our group, and in which disease evolves either across disparities in major histocompatibility complex (MCH) class I and II, minor histocompatibility antigens (miHags) or both. Metabolomics changes following syngeneic and allogeneic HCT at post-transplantation Days +7 (cytokine storm phase) and Days +42 (cellular effector phase) are compared to baseline wild-type (naive) controls. Prior to analysis, naïve - and experimental mice (N=3 from each group) were fed with semi-liquid diet supplemented with tracers (13C6-glucose ) over 24 hours. At the end of 7 days or 42 days, respectively, feces and aGVHD target organs (colon, liver and lung) were collected from all groups and further processed and / or analyzed. We expect to reveal metabolic pathways affected after allo-HCT which contribute to immune cell mediated lung injury (IPS) and will potentially identify different metabolic pathways in other GVHD target organs.
Institute
University of Kentucky
DepartmentMCC
Last NameHildebrandt
First NameGerhard
AddressCTW-453, 900 South Limestone street. UKY. Lexington, Kentucky-40536
Emailgerhard.hildebrandt@uky.edu
Phone800-333-8874
Submit Date2020-08-12
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailMS(Dir. Inf.)
Release Date2020-09-10
Release Version1
Gerhard Hildebrandt Gerhard Hildebrandt
https://dx.doi.org/10.21228/M8BH7T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Protocol
SA12378408_C1-2_Colon_allogenic_42days_170427_UKy_GCH_rep2-lipidallogenic
SA12378507_C1-1_Colon_allogenic_42days_170427_UKy_GCH_rep1-lipidallogenic
SA12378613_C1-1_Colon_allogenic_7days_170427_UKy_GCH_rep1-lipidallogenic
SA12378715_C1-20_Colon_allogenic_7days_170427_UKy_GCH_rep3-lipidallogenic
SA12378814_C1-2_Colon_allogenic_7days_170427_UKy_GCH_rep2-lipidallogenic
SA12378909_C2-0_Colon_allogenic_42days_170427_UKy_GCH_rep1-lipidallogenic
SA12379001_A0_Colon_naive_0days_170427_UKy_GCH_rep1-lipidnaive
SA12379103_A2_Colon_naive_0days_170427_UKy_GCH_rep3-lipidnaive
SA12379202_A1_Colon_naive_0days_170427_UKy_GCH_rep2-lipidnaive
SA12379312_B1-2_Colon_syngenic_7days_170427_UKy_GCH_rep3-lipidsyngenic
SA12379410_B1-0_Colon_syngenic_7days_170427_UKy_GCH_rep1-lipidsyngenic
SA12379511_B1-1_Colon_syngenic_7days_170427_UKy_GCH_rep2-lipidsyngenic
SA12379605_B1_Colon_syngenic_42days_170427_UKy_GCH_rep2-lipidsyngenic
SA12379704_B0_Colon_syngenic_42days_170427_UKy_GCH_rep1-lipidsyngenic
SA12379806_B2_Colon_syngenic_42days_170427_UKy_GCH_rep3-lipidsyngenic
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