Summary of Study ST001455

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000993. The data can be accessed directly via it's Project DOI: 10.21228/M8BH7T This work is supported by NIH grant, U2C- DK119886.

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Study IDST001455
Study TitleMetabolomics of lung injury after allogeneic hematopoietic cell transplantation - Liver NMR 1D
Study Typepreliminary data
Study SummaryAllogeneic hematopoietic cell transplantation (allo-HCT) is a potentially curative treatment option for a variety of hematological malignancies. Interactions between the donor immune system and the patient tissue result in a disease, called GVHD. The pathophysiology of acute GVHD can be hypothesized in three sequential phases: cytokine storm and activation of the antigen-presenting cells (APC), donor T cell activation and effector cell phase. Idiopathic pneumonia syndrome (IPS) is one of the most deleterious complications after allogeneic HCT and is considered not only to be related to conditioning regimen toxicity but also represents an end organ damage caused by allo-reactive T cells, therefore making the lung susceptible to a two-pronged attack, one of which overlaps with GVHD causing other target organ injury. IPS results in mortality of up to 90% of patients. We will use a murine model of IPS and GVHD which is well established in our group, and in which disease evolves either across disparities in major histocompatibility complex (MCH) class I and II, minor histocompatibility antigens (miHags) or both. Metabolomics changes following syngeneic and allogeneic HCT at post-transplantation Days +7 (cytokine storm phase) and Days +42 (cellular effector phase) are compared to baseline wild-type (naive) controls. Prior to analysis, naïve - and experimental mice (N=3 from each group) were fed with semi-liquid diet supplemented with tracers (13C6-glucose ) over 24 hours. At the end of 7 days or 42 days, respectively, feces and aGVHD target organs (colon, liver and lung) were collected from all groups and further processed and / or analyzed. We expect to reveal metabolic pathways affected after allo-HCT which contribute to immune cell mediated lung injury (IPS) and will potentially identify different metabolic pathways in other GVHD target organs.
Institute
University of Kentucky
DepartmentMCC
Last NameHildebrandt
First NameGerhard
AddressCTW-453, 900 South Limestone street. UKY. Lexington, Kentucky-40536
Emailgerhard.hildebrandt@uky.edu
Phone800-333-8874
Submit Date2020-08-21
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2020-09-10
Release Version1
Gerhard Hildebrandt Gerhard Hildebrandt
https://dx.doi.org/10.21228/M8BH7T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Protocol
SA12414815_C1-20_Liver_allogenic_7days_170427_UKy_GCH_rep3-polar-NMR_Aallogenic
SA12414913_C1-1_Liver_allogenic_7days_170427_UKy_GCH_rep1-polar-NMR_Aallogenic
SA12415007_C1-1_Liver_allogenic_42days_170427_UKy_GCH_rep1-polar-NMR_Aallogenic
SA12415108_C1-2_Liver_allogenic_42days_170427_UKy_GCH_rep2-polar-NMR_Aallogenic
SA12415209_C2-0_Liver_allogenic_42days_170427_UKy_GCH_rep1-polar-NMR_Aallogenic
SA12415314_C1-2_Liver_allogenic_7days_170427_UKy_GCH_rep2-polar-NMR_Aallogenic
SA12415402_A1_Liver_naive_0days_170427_UKy_GCH_rep2-polar-NMR_Anaive
SA12415501_A0_Liver_naive_0days_170427_UKy_GCH_rep1-polar-NMR_Anaive
SA12415603_A2_Liver_naive_0days_170427_UKy_GCH_rep3-polar-NMR_Anaive
SA12415704_B0_Liver_syngenic_42days_170427_UKy_GCH_rep1-polar-NMR_Asyngenic
SA12415805_B1_Liver_syngenic_42days_170427_UKy_GCH_rep2-polar-NMR_Asyngenic
SA12415910_B1-0_Liver_syngenic_7days_170427_UKy_GCH_rep1-polar-NMR_Asyngenic
SA12416011_B1-1_Liver_syngenic_7days_170427_UKy_GCH_rep2-polar-NMR_Asyngenic
SA12416112_B1-2_Liver_syngenic_7days_170427_UKy_GCH_rep3-polar-NMR_Asyngenic
SA12416206_B2_Liver_syngenic_42days_170427_UKy_GCH_rep3-polar-NMR_Asyngenic
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