Summary of Study ST001704
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001090. The data can be accessed directly via it's Project DOI: 10.21228/M8T12F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001704 |
Study Title | Sclerostin antibody increases trabecular bone and bone mechanical properties by increasing osteoblast activity damaged by whole-body irradiation in mice |
Study Type | Basic research |
Study Summary | Irradiation therapy causes bone deterioration and increased risk for skeletal-related events. Irradiation interferes with trabecular architecture through increased osteoclastic activity, decreased osteoblastic activity, and increased adipocyte expansion in the bone marrow (BM), which further compounds bone-related disease. Neutralizing antibodies to sclerostin (Scl-Ab) increase bone mass and strength by increasing bone formation and reducing bone resorption. We hypothesized that treatment with Scl-Ab would attenuate the adverse effects of irradiation by increasing bone volume and decreasing BM adipose tissue (BMAT), resulting in better quality bone. In this study, 12-week-old female C57BL/6J mice were exposed to 6 Gy whole-body irradiation or were non-irradiated, then administered Scl-Ab (25 mg/kg) or vehicle weekly for 5 weeks. Femoral µCT analysis confirmed that the overall effect of IR significantly decreased trabecular bone volume/total volume (Tb.BV/TV) (2-way ANOVA, p<0.0001) with a -43.8% loss in Tb.BV/TV in the IR control group. Scl-Ab independently increased Tb.BV/TV by 3.07-fold in non-irradiated and 3.6-fold in irradiated mice (2-way ANOVA, p<0.0001). Irradiation did not affect cortical parameters, although Scl-Ab increased cortical thickness and area significantly in both irradiated and non-irradiated mice (2-way ANOVA, p<0.0001). Femoral mechanical testing confirmed Scl-Ab significantly increased bending rigidity and ultimate moment independently of irradiation (2-way ANOVA, p<0.0001). Static and dynamic histomorphometry of the femoral metaphysis revealed osteoblast vigor, not number, was significantly increased in the irradiated mice treated with Scl-Ab. Systemic alterations were assessed through serum lipidomic analysis, which showed that Scl-Ab normalized lipid profiles in the irradiated group. This data supports the theory of sclerostin as a novel contributor to the regulation of osteoblast activity after irradiation. Overall, our data support the hypothesis that Scl-Ab ameliorates the deleterious effects of whole-body irradiation on bone and adipose tissue in a mouse model. Our findings suggest that future research into localized and systemic therapies after irradiation exposure is warranted. |
Institute | Mainehealth |
Last Name | Vary |
First Name | Calvin |
Address | 81 Research Drive, Scarborough, ME, USA 04074 |
varyc@mmc.org | |
Phone | 2073968148 |
Submit Date | 2021-02-10 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Analysis Type Detail | MS(Dir. Inf.) |
Release Date | 2021-03-01 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
mb_sample_id | local_sample_id | Treatment |
---|---|---|
SA158357 | negMSMSALL-SC-D35-IR_C101_1 | IR Control |
SA158358 | negMSMSALL-SC-D35-IR_C101_2 | IR Control |
SA158359 | negMSMSALL-SC-D35-IR_C100_2 | IR Control |
SA158360 | negMSMSALL-SC-D3-IR_C100_2 | IR Control |
SA158361 | negMSMSALL-SC-D3-IR_C101_2 | IR Control |
SA158362 | negMSMSALL-SC-D3-IR_C107_1 | IR Control |
SA158363 | negMSMSALL-SC-D3-IR_C107_2 | IR Control |
SA158364 | negMSMSALL-SC-D3-IR_C101_1 | IR Control |
SA158365 | posMSMSALL-SC-D35-IR_C100_1 | IR Control |
SA158366 | negMSMSALL-SC-D35-IR_C107_2 | IR Control |
SA158367 | negMSMSALL-SC-D3-IR_C100_1 | IR Control |
SA158368 | negMSMSALL-SC-D35-IR_C107_1 | IR Control |
SA158369 | negMSMSALL-SC-D35-IR_C100_1 | IR Control |
SA158370 | posMSMSALL-SC-D3-IR_C107_1 | IR Control |
SA158371 | posMSMSALL-SC-D3-IR_C107_2 | IR Control |
SA158372 | posMSMSALL-SC-D35-IR_C107_1 | IR Control |
SA158373 | posMSMSALL-SC-D3-IR_C101_2 | IR Control |
SA158374 | posMSMSALL-SC-D3-IR_C101_1 | IR Control |
SA158375 | posMSMSALL-SC-D3-IR_C100_1 | IR Control |
SA158376 | posMSMSALL-SC-D3-IR_C100_2 | IR Control |
SA158377 | posMSMSALL-SC-D35-IR_C101_2 | IR Control |
SA158378 | posMSMSALL-SC-D35-IR_C107_2 | IR Control |
SA158379 | posMSMSALL-SC-D35-IR_C100_2 | IR Control |
SA158380 | posMSMSALL-SC-D35-IR_C101_1 | IR Control |
SA158333 | posMSMSALL-SC-D3-IR_C133_2 | IR + Scl-Ab |
SA158334 | negMSMSALL-SC-D35-IR_C136_2 | IR + Scl-Ab |
SA158335 | posMSMSALL-SC-D3-IR_C135_1 | IR + Scl-Ab |
SA158336 | posMSMSALL-SC-D3-IR_C135_2 | IR + Scl-Ab |
SA158337 | posMSMSALL-SC-D3-IR_C136_1 | IR + Scl-Ab |
SA158338 | negMSMSALL-SC-D35-IR_C136_1 | IR + Scl-Ab |
SA158339 | negMSMSALL-SC-D35-IR_C135_2 | IR + Scl-Ab |
SA158340 | posMSMSALL-SC-D3-IR_C133_1 | IR + Scl-Ab |
SA158341 | negMSMSALL-SC-D35-IR_C133_1 | IR + Scl-Ab |
SA158342 | negMSMSALL-SC-D35-IR_C133_2 | IR + Scl-Ab |
SA158343 | negMSMSALL-SC-D35-IR_C135_1 | IR + Scl-Ab |
SA158344 | posMSMSALL-SC-D3-IR_C136_2 | IR + Scl-Ab |
SA158345 | negMSMSALL-SC-D3-IR_C133_1 | IR + Scl-Ab |
SA158346 | negMSMSALL-SC-D3-IR_C135_1 | IR + Scl-Ab |
SA158347 | posMSMSALL-SC-D35-IR_C133_1 | IR + Scl-Ab |
SA158348 | negMSMSALL-SC-D3-IR_C135_2 | IR + Scl-Ab |
SA158349 | negMSMSALL-SC-D3-IR_C136_1 | IR + Scl-Ab |
SA158350 | negMSMSALL-SC-D3-IR_C136_2 | IR + Scl-Ab |
SA158351 | posMSMSALL-SC-D35-IR_C133_2 | IR + Scl-Ab |
SA158352 | posMSMSALL-SC-D35-IR_C135_1 | IR + Scl-Ab |
SA158353 | negMSMSALL-SC-D3-IR_C133_2 | IR + Scl-Ab |
SA158354 | posMSMSALL-SC-D35-IR_C136_1 | IR + Scl-Ab |
SA158355 | posMSMSALL-SC-D35-IR_C136_2 | IR + Scl-Ab |
SA158356 | posMSMSALL-SC-D35-IR_C135_2 | IR + Scl-Ab |
SA158405 | negMSMSALL-SC-D3-IR_SA121_2 | Non-IR Control |
SA158406 | posMSMSALL-SC-D3-IR_SA123_2 | Non-IR Control |
SA158407 | negMSMSALL-SC-D3-IR_SA123_1 | Non-IR Control |
SA158408 | negMSMSALL-SC-D3-IR_SA121_1 | Non-IR Control |
SA158409 | negMSMSALL-SC-D3-IR_SA127_2 | Non-IR Control |
SA158410 | posMSMSALL-SC-D3-IR_SA127_2 | Non-IR Control |
SA158411 | posMSMSALL-SC-D3-IR_SA127_1 | Non-IR Control |
SA158412 | negMSMSALL-SC-D3-IR_SA127_1 | Non-IR Control |
SA158413 | negMSMSALL-SC-D3-IR_SA123_2 | Non-IR Control |
SA158414 | posMSMSALL-SC-D35-IR_SA121_1 | Non-IR Control |
SA158415 | negMSMSALL-SC-D35-IR_SA121_2 | Non-IR Control |
SA158416 | negMSMSALL-SC-D35-IR_SA121_1 | Non-IR Control |
SA158417 | posMSMSALL-SC-D3-IR_SA123_1 | Non-IR Control |
SA158418 | negMSMSALL-SC-D35-IR_SA123_1 | Non-IR Control |
SA158419 | negMSMSALL-SC-D35-IR_SA127_1 | Non-IR Control |
SA158420 | negMSMSALL-SC-D35-IR_SA127_2 | Non-IR Control |
SA158421 | posMSMSALL-SC-D35-IR_SA127_2 | Non-IR Control |
SA158422 | posMSMSALL-SC-D35-IR_SA127_1 | Non-IR Control |
SA158423 | negMSMSALL-SC-D35-IR_SA123_2 | Non-IR Control |
SA158424 | posMSMSALL-SC-D3-IR_SA121_1 | Non-IR Control |
SA158425 | posMSMSALL-SC-D35-IR_SA121_2 | Non-IR Control |
SA158426 | posMSMSALL-SC-D35-IR_SA123_1 | Non-IR Control |
SA158427 | posMSMSALL-SC-D35-IR_SA123_2 | Non-IR Control |
SA158428 | posMSMSALL-SC-D3-IR_SA121_2 | Non-IR Control |
SA158381 | posMSMSALL-SC-D35-IR_SA152_2 | Non-IR + Scl-Ab |
SA158382 | posMSMSALL-SC-D35-IR_SA152_1 | Non-IR + Scl-Ab |
SA158383 | negMSMSALL-SC-D35-IR_SA158_1 | Non-IR + Scl-Ab |
SA158384 | negMSMSALL-SC-D35-IR_SA153_1 | Non-IR + Scl-Ab |
SA158385 | negMSMSALL-SC-D35-IR_SA153_2 | Non-IR + Scl-Ab |
SA158386 | negMSMSALL-SC-D35-IR_SA158_2 | Non-IR + Scl-Ab |
SA158387 | negMSMSALL-SC-D3-IR_SA158_2 | Non-IR + Scl-Ab |
SA158388 | negMSMSALL-SC-D35-IR_SA152_1 | Non-IR + Scl-Ab |
SA158389 | negMSMSALL-SC-D3-IR_SA152_1 | Non-IR + Scl-Ab |
SA158390 | negMSMSALL-SC-D3-IR_SA152_2 | Non-IR + Scl-Ab |
SA158391 | negMSMSALL-SC-D3-IR_SA153_1 | Non-IR + Scl-Ab |
SA158392 | negMSMSALL-SC-D3-IR_SA153_2 | Non-IR + Scl-Ab |
SA158393 | negMSMSALL-SC-D3-IR_SA158_1 | Non-IR + Scl-Ab |
SA158394 | negMSMSALL-SC-D35-IR_SA152_2 | Non-IR + Scl-Ab |
SA158395 | posMSMSALL-SC-D3-IR_SA158_2 | Non-IR + Scl-Ab |
SA158396 | posMSMSALL-SC-D35-IR_SA153_2 | Non-IR + Scl-Ab |
SA158397 | posMSMSALL-SC-D35-IR_SA158_2 | Non-IR + Scl-Ab |
SA158398 | posMSMSALL-SC-D3-IR_SA153_2 | Non-IR + Scl-Ab |
SA158399 | posMSMSALL-SC-D3-IR_SA158_1 | Non-IR + Scl-Ab |
SA158400 | posMSMSALL-SC-D35-IR_SA153_1 | Non-IR + Scl-Ab |
SA158401 | posMSMSALL-SC-D35-IR_SA158_1 | Non-IR + Scl-Ab |
SA158402 | posMSMSALL-SC-D3-IR_SA152_1 | Non-IR + Scl-Ab |
SA158403 | posMSMSALL-SC-D3-IR_SA152_2 | Non-IR + Scl-Ab |
SA158404 | posMSMSALL-SC-D3-IR_SA153_1 | Non-IR + Scl-Ab |
Showing results 1 to 96 of 96 |