Summary of Study ST001725
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001105. The data can be accessed directly via it's Project DOI: 10.21228/M8VT4F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001725 |
| Study Title | Lipidomics dataset of Danio rerio optic nerve regeneration model |
| Study Summary | The right optic nerve of 1 year old female and male Danio rerio were crushed and collected three days after. Matching controls of uninjured eyes were also collected. The tissue was dissected from euthanized fish and “flash” frozen on dry ice in Eppendorf tubes. Due to the small size of the nerves, for each category (female crush, female control, male crush, male control) n=24 the samples were pooled. The brain from one male fish was also collected for control/calibration. Lipid extraction was done with the Bligh and Dyer [2] method, followed by untargeted liquid chromatography-mass spectrometry (LC MS-MS) lipid profiling using a Q-Exactive Orbitrap instrument coupled with Vanquish Horizon Binary UHPLC LC-MS system. The lipids were identified and quantified with LipidSearch 4.2.21 and the statistical analysis was conducted through Metaboanalyst 5.0. |
| Institute | University of Miami |
| Last Name | Bhattacharya |
| First Name | Sanjoy |
| Address | 1638 NW 10th Avenue, Room 706-A, Miami, FL 33136 |
| sbhattacharya@med.miami.edu | |
| Phone | 13054824103 |
| Submit Date | 2021-03-12 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-03-31 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Fish; Subject species: Danio rerio (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample Source |
|---|---|---|
| SA162368 | Brain_02 | Brain |
| SA162369 | Brain_01 | Brain |
| SA162370 | Brain_04 | Brain |
| SA162371 | Brain_03 | Brain |
| SA162372 | Male_Control_04 | Control |
| SA162373 | Female_Control_01 | Control |
| SA162374 | Female_Control_04 | Control |
| SA162375 | Female_Control_03 | Control |
| SA162376 | Female_Control_02 | Control |
| SA162377 | Male_Control_03 | Control |
| SA162378 | Male_Control_02 | Control |
| SA162379 | Male_Control_01 | Control |
| SA162380 | Female_Crush_03 | Crush |
| SA162381 | Male_Crush_01 | Crush |
| SA162382 | Female_Crush_02 | Crush |
| SA162383 | Female_Crush_04 | Crush |
| SA162384 | Male_Crush_02 | Crush |
| SA162385 | Male_Crush_04 | Crush |
| SA162386 | Male_Crush_03 | Crush |
| SA162387 | Female_Crush_01 | Crush |
| Showing results 1 to 20 of 20 |
