Summary of Study ST002055
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001300. The data can be accessed directly via it's Project DOI: 10.21228/M8P69K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST002055 |
| Study Title | Metabolomic Profiling of Human Pluripotent Stem Cell Differentiation into Lung Progenitors |
| Study Summary | Metabolism is vital to cellular function and tissue homeostasis during human lung development. In utero, embryonic pluripotent stem cells undergo endodermal differentiation towards a lung progenitor cell fate that can be mimicked in vitro using induced human pluripotent stem cells (hiPSCs) to study genetic mutations. To identify differences between wild type and surfactant protein B (SFTPB)-deficient cell lines during endoderm specification towards lung, we used an untargeted metabolomics approach to evaluate the developmental changes in metabolites. We found that the metabolites most enriched during the differentiation from pluripotent stem cell to lung progenitor cell, regardless of cell line, were sphingomyelins and phosphatidylcholines, two important lipid classes in fetal lung development. The SFTPB mutation had no metabolic impact on early endodermal lung development. The identified metabolite signatures during lung progenitor cell differentiation may be utilized as biomarkers for normal embryonic lung development. |
| Institute | The Hospital for Sick Children |
| Last Name | Post |
| First Name | Martin |
| Address | 555 University Avenue |
| martin.post@sickkids.ca | |
| Phone | 4168136772 |
| Submit Date | 2021-06-29 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS/MS(Dir. Inf.) |
| Release Date | 2022-01-13 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Differentiation stage |
|---|---|---|
| SA193726 | 60 - ICE 12 | Anterior Foregut Endoderm |
| SA193727 | 28 - NCRM1 12 | Anterior Foregut Endoderm |
| SA193728 | 26 - NCRM1 10 | Anterior Foregut Endoderm |
| SA193729 | 25 - NCRM1 9 | Anterior Foregut Endoderm |
| SA193730 | 59 - ICE 11 | Anterior Foregut Endoderm |
| SA193731 | 42 - ICD 10 | Anterior Foregut Endoderm |
| SA193732 | 57 - ICE 9 | Anterior Foregut Endoderm |
| SA193733 | 58 - ICE 10 | Anterior Foregut Endoderm |
| SA193734 | 44 - ICD 12 | Anterior Foregut Endoderm |
| SA193735 | 43 - ICD 11 | Anterior Foregut Endoderm |
| SA193736 | 41 - ICD 9 | Anterior Foregut Endoderm |
| SA193737 | 27 - NCRM1 11 | Anterior Foregut Endoderm |
| SA193738 | 10 - CA1 10 | Anterior Foregut Endoderm |
| SA193739 | 9 - CA1 9 | Anterior Foregut Endoderm |
| SA193740 | 11 - CA1 11 | Anterior Foregut Endoderm |
| SA193741 | 12 - CA1 12 | Anterior Foregut Endoderm |
| SA193742 | 7 - CA1 7 | Definitive Endoderm |
| SA193743 | 56 - ICE 8 | Definitive Endoderm |
| SA193744 | 6 - CA1 6 | Definitive Endoderm |
| SA193745 | 40 - ICD 8 | Definitive Endoderm |
| SA193746 | 39 - ICD 7 | Definitive Endoderm |
| SA193747 | 37 - ICD 5 | Definitive Endoderm |
| SA193748 | 8 - CA1 8 | Definitive Endoderm |
| SA193749 | 55 - ICE 7 | Definitive Endoderm |
| SA193750 | 5 - CA1 5 | Definitive Endoderm |
| SA193751 | 54 - ICE 6 | Definitive Endoderm |
| SA193752 | 22 - NCRM1 6 | Definitive Endoderm |
| SA193753 | 21 - NCRM1 5 | Definitive Endoderm |
| SA193754 | 53 - ICE 5 | Definitive Endoderm |
| SA193755 | 23 - NCRM1 7 | Definitive Endoderm |
| SA193756 | 24 - NCRM1 8 | Definitive Endoderm |
| SA193757 | 38 - ICD 6 | Definitive Endoderm |
| SA193778 | 33 - ICD 1 | iPSC |
| SA193779 | 20 - NCRM1 4 | iPSC |
| SA193780 | 19 - NCRM1 3 | iPSC |
| SA193781 | 18 - NCRM1 2 | iPSC |
| SA193782 | 17 - NCRM1 1 | iPSC |
| SA193783 | 34 - ICD 2 | iPSC |
| SA193784 | 35 - ICD 3 | iPSC |
| SA193785 | 51 - ICE 3 | iPSC |
| SA193786 | 50 - ICE 2 | iPSC |
| SA193787 | 49 - ICE 1 | iPSC |
| SA193788 | 36 - ICD 4 | iPSC |
| SA193789 | 52 - ICE 4 | iPSC |
| SA193758 | 45 - ICD 13 | Lung Progenitor Cell |
| SA193759 | 46 - ICD 14 | Lung Progenitor Cell |
| SA193760 | 47 - ICD 15 | Lung Progenitor Cell |
| SA193761 | 48 - ICD 16 | Lung Progenitor Cell |
| SA193762 | 31 - NCRM1 15 | Lung Progenitor Cell |
| SA193763 | 64 - ICE 16 | Lung Progenitor Cell |
| SA193764 | 13 - CA1 13 | Lung Progenitor Cell |
| SA193765 | 16 - CA1 16 | Lung Progenitor Cell |
| SA193766 | 15 - CA1 15 | Lung Progenitor Cell |
| SA193767 | 63 - ICE 15 | Lung Progenitor Cell |
| SA193768 | 62 - ICE 14 | Lung Progenitor Cell |
| SA193769 | 14 - CA1 14 | Lung Progenitor Cell |
| SA193770 | 32 - NCRM1 16 | Lung Progenitor Cell |
| SA193771 | 30 - NCRM1 14 | Lung Progenitor Cell |
| SA193772 | 29 - NCRM1 13 | Lung Progenitor Cell |
| SA193773 | 61 - ICE 13 | Lung Progenitor Cell |
| SA193774 | 1 - CA1 1 | PSC |
| SA193775 | 4 - CA1 4 | PSC |
| SA193776 | 3 - CA1 3 | PSC |
| SA193777 | 2 - CA1 2 | PSC |
| Showing results 1 to 64 of 64 |
