Summary of Study ST002055

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001300. The data can be accessed directly via it's Project DOI: 10.21228/M8P69K This work is supported by NIH grant, U2C- DK119886.


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Study IDST002055
Study TitleMetabolomic Profiling of Human Pluripotent Stem Cell Differentiation into Lung Progenitors
Study SummaryMetabolism is vital to cellular function and tissue homeostasis during human lung development. In utero, embryonic pluripotent stem cells undergo endodermal differentiation towards a lung progenitor cell fate that can be mimicked in vitro using induced human pluripotent stem cells (hiPSCs) to study genetic mutations. To identify differences between wild type and surfactant protein B (SFTPB)-deficient cell lines during endoderm specification towards lung, we used an untargeted metabolomics approach to evaluate the developmental changes in metabolites. We found that the metabolites most enriched during the differentiation from pluripotent stem cell to lung progenitor cell, regardless of cell line, were sphingomyelins and phosphatidylcholines, two important lipid classes in fetal lung development. The SFTPB mutation had no metabolic impact on early endodermal lung development. The identified metabolite signatures during lung progenitor cell differentiation may be utilized as biomarkers for normal embryonic lung development.
The Hospital for Sick Children
Last NamePost
First NameMartin
Address555 University Avenue
Submit Date2021-06-29
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2022-01-13
Release Version1
Martin Post Martin Post application/zip

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Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Differentiation stage
SA19372660 - ICE 12Anterior Foregut Endoderm
SA19372728 - NCRM1 12Anterior Foregut Endoderm
SA19372826 - NCRM1 10Anterior Foregut Endoderm
SA19372925 - NCRM1 9Anterior Foregut Endoderm
SA19373059 - ICE 11Anterior Foregut Endoderm
SA19373142 - ICD 10Anterior Foregut Endoderm
SA19373257 - ICE 9Anterior Foregut Endoderm
SA19373358 - ICE 10Anterior Foregut Endoderm
SA19373444 - ICD 12Anterior Foregut Endoderm
SA19373543 - ICD 11Anterior Foregut Endoderm
SA19373641 - ICD 9Anterior Foregut Endoderm
SA19373727 - NCRM1 11Anterior Foregut Endoderm
SA19373810 - CA1 10Anterior Foregut Endoderm
SA1937399 - CA1 9Anterior Foregut Endoderm
SA19374011 - CA1 11Anterior Foregut Endoderm
SA19374112 - CA1 12Anterior Foregut Endoderm
SA1937427 - CA1 7Definitive Endoderm
SA19374356 - ICE 8Definitive Endoderm
SA1937446 - CA1 6Definitive Endoderm
SA19374540 - ICD 8Definitive Endoderm
SA19374639 - ICD 7Definitive Endoderm
SA19374737 - ICD 5Definitive Endoderm
SA1937488 - CA1 8Definitive Endoderm
SA19374955 - ICE 7Definitive Endoderm
SA1937505 - CA1 5Definitive Endoderm
SA19375154 - ICE 6Definitive Endoderm
SA19375222 - NCRM1 6Definitive Endoderm
SA19375321 - NCRM1 5Definitive Endoderm
SA19375453 - ICE 5Definitive Endoderm
SA19375523 - NCRM1 7Definitive Endoderm
SA19375624 - NCRM1 8Definitive Endoderm
SA19375738 - ICD 6Definitive Endoderm
SA19377833 - ICD 1iPSC
SA19377920 - NCRM1 4iPSC
SA19378019 - NCRM1 3iPSC
SA19378118 - NCRM1 2iPSC
SA19378217 - NCRM1 1iPSC
SA19378334 - ICD 2iPSC
SA19378435 - ICD 3iPSC
SA19378551 - ICE 3iPSC
SA19378650 - ICE 2iPSC
SA19378749 - ICE 1iPSC
SA19378836 - ICD 4iPSC
SA19378952 - ICE 4iPSC
SA19375845 - ICD 13Lung Progenitor Cell
SA19375946 - ICD 14Lung Progenitor Cell
SA19376047 - ICD 15Lung Progenitor Cell
SA19376148 - ICD 16Lung Progenitor Cell
SA19376231 - NCRM1 15Lung Progenitor Cell
SA19376364 - ICE 16Lung Progenitor Cell
SA19376413 - CA1 13Lung Progenitor Cell
SA19376516 - CA1 16Lung Progenitor Cell
SA19376615 - CA1 15Lung Progenitor Cell
SA19376763 - ICE 15Lung Progenitor Cell
SA19376862 - ICE 14Lung Progenitor Cell
SA19376914 - CA1 14Lung Progenitor Cell
SA19377032 - NCRM1 16Lung Progenitor Cell
SA19377130 - NCRM1 14Lung Progenitor Cell
SA19377229 - NCRM1 13Lung Progenitor Cell
SA19377361 - ICE 13Lung Progenitor Cell
SA1937741 - CA1 1PSC
SA1937754 - CA1 4PSC
SA1937763 - CA1 3PSC
SA1937772 - CA1 2PSC
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