Summary of Study ST002220

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001418. The data can be accessed directly via it's Project DOI: 10.21228/M8F409 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002220
Study TitleCatabolism of branched-chain amino acids (BCAAs) in renal cells HK2 and 786-O
Study SummaryThe objective of this experiment is to compare the catabolism of branched-chain amino acids (BCAAs) in human renal epithelial cell line HK2 versus ccRCC cell lines 786-O, 786-M1A and 786-M2A using 13C6-labelled leucine and isoleucine stable isotope tracers. To this end, we incubated the above cell lines with 13C6-leucine and 13C6-isoleucine in Plasmax media for 27h. Data were generated from 5 independent cultures. This is Part I of the study and the experimental number is MS42.
Institute
CECAD Research Center
Last NameYang
First NameMing
AddressJoseph-Stelzmann-Straße 26, CECAD Research Center, Köln, Koeln, 50931, Germany
Emailming.yang@uni-koeln.de
Phone+4922147884306
Submit Date2022-07-15
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-08-03
Release Version1
Ming Yang Ming Yang
https://dx.doi.org/10.21228/M8F409
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Cell line Treatment
SA211952MS42-13786-M1A 13C6-leucine+13C6-isoleucine
SA211953MS42-14786-M1A 13C6-leucine+13C6-isoleucine
SA211954MS42-15786-M1A 13C6-leucine+13C6-isoleucine
SA211955MS42-12786-M1A 13C6-leucine+13C6-isoleucine
SA211956MS42-11786-M1A 13C6-leucine+13C6-isoleucine
SA211957MS42-18786-M2A 13C6-leucine+13C6-isoleucine
SA211958MS42-19786-M2A 13C6-leucine+13C6-isoleucine
SA211959MS42-16786-M2A 13C6-leucine+13C6-isoleucine
SA211960MS42-20786-M2A 13C6-leucine+13C6-isoleucine
SA211961MS42-17786-M2A 13C6-leucine+13C6-isoleucine
SA211962MS42-07786-O 13C6-leucine+13C6-isoleucine
SA211963MS42-06786-O 13C6-leucine+13C6-isoleucine
SA211964MS42-08786-O 13C6-leucine+13C6-isoleucine
SA211965MS42-09786-O 13C6-leucine+13C6-isoleucine
SA211966MS42-10786-O 13C6-leucine+13C6-isoleucine
SA211967MS42-02HK2 13C6-leucine+13C6-isoleucine
SA211968MS42-04HK2 13C6-leucine+13C6-isoleucine
SA211969MS42-01HK2 13C6-leucine+13C6-isoleucine
SA211970MS42-05HK2 13C6-leucine+13C6-isoleucine
SA211971MS42-03HK2 13C6-leucine+13C6-isoleucine
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