Summary of Study ST002979
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001855. The data can be accessed directly via it's Project DOI: 10.21228/M8XT6T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002979 |
| Study Title | Untargeted lipidomics profiling of TSC microglia |
| Study Summary | In this study, human microglia from induced pluripotent stem cells (iPSCs) derived from a TSC patient cohort were generated . With a comprehensively molecular and cellular characterization on TSC microglia, including transcriptomics, proteomics/phosphopreteomics, and lipidomics, patient-carrying TSC2 mutations lead to aberrant lipid metabolism were found, in particular, upregulated glycerophosphocholines and fatty acyls in TSC microglia, resulting in increased phagocytosis and inflammation. Strikingly, the dysregulated lipid metabolism in TSC microglia is driven by hyper-activation of mTOR-LPL pathway. Furthermore, cellular and electrophysiological assessments of neuron/microglia co-cultures revealed that TSC microglia directly affect neuronal development and excitability as well as neuronal network activity, which could be largely ameliorated by mTOR/LPL inhibition |
| Institute | St Jude Children's Research Hospital |
| Last Name | Xie |
| First Name | Boer |
| Address | 262 Danny Thomas Place, Memphis, TN, 38105, USA |
| xbr429@gmail.com | |
| Phone | (901) 595-7499 |
| Submit Date | 2023-11-09 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-11-17 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype |
|---|---|---|
| SA323369 | TSC10-3 | TSC mutation |
| SA323370 | TSC10-1 | TSC mutation |
| SA323371 | TSC7-3 | TSC mutation |
| SA323372 | TSC10-Homo-1 | TSC mutation |
| SA323373 | TSC10-Homo-2 | TSC mutation |
| SA323374 | TSC7-4 | TSC mutation |
| SA323375 | TSC10-Homo-3 | TSC mutation |
| SA323376 | TSC7-2 | TSC mutation |
| SA323377 | TSC10-2 | TSC mutation |
| SA323378 | TSC7-1 | TSC mutation |
| SA323379 | 12C1-4 | wildtype |
| SA323380 | 12C1-3 | wildtype |
| SA323381 | C1-2-4 | wildtype |
| SA323382 | 426-4 | wildtype |
| SA323383 | C1-2-2 | wildtype |
| SA323384 | C1-2-3 | wildtype |
| SA323385 | PGP1-4 | wildtype |
| SA323386 | 12C1-2 | wildtype |
| SA323387 | 12C1-1 | wildtype |
| SA323388 | PGP1-2 | wildtype |
| SA323389 | PGP1-3 | wildtype |
| SA323390 | C1-2-1 | wildtype |
| SA323391 | PGP1-1 | wildtype |
| SA323392 | 426-2 | wildtype |
| SA323393 | 426-3 | wildtype |
| SA323394 | 426-1 | wildtype |
| Showing results 1 to 26 of 26 |
