Summary of Study ST003399

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002105. The data can be accessed directly via it's Project DOI: 10.21228/M8HV52 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003399
Study TitleChemical Biology Meets Metabolomics: The Response of Barley Seedlings to 3,5-Dichloroanthranilic Acid, a Resistance Inducer
Study TypePlant metabolomics
Study SummaryAdvances in combinatorial synthesis and high-throughput screening methods have led to renewed interest in synthetic plant immune activators as well as priming agents. 3,5-Dichloroanthranilic acid (3,5-DCAA) is a derivative of anthranilic acid that have shown potency in activating defence mechanisms in Arabidopsis and barley plants. Chemical biology which is the interface of chemistry and biology can make use of metabolomics approaches and tools to better understand molecular mechanisms operating in complex biological systems. Aim: Here we report on the untargeted metabolomics profiling of barley seedlings treated with 3,5-DCAA to gain deeper insights into the mechanism of action of this resistance inducer. Methodology: Hydro-methanolic extracts from different time periods (12, 24 and 36 h post-treatment) were analysed on ultra-high performance liquid chromatography hyphenated with a high-resolution mass spectrometer. Both unsupervised and supervised chemometric methods were used to reveal hidden patterns and highlight metabolite variables associated with the treatment. Results: Based on the metabolites identified, both the phenylpropanoid and octadecanoid pathways appear to be main route activated by 3,5-DCAA. Different classes of responsive metabolites were annotated with favonoids, more especially flavones, the most dominant. Given the limited understanding of this inducer, this study offers a metabolomics analysis of the response triggered by its foliar application in barley. This additional insight could help make informed decision for the development of more effective strategies for crop protection and improvement, ultimately contributing to agricultural sustainability and resilience.
Institute
University of Johannesburg
DepartmentBiochemistry
LaboratoryPlant metabolomics
Last NameClaude Yasmine Hamany Djande
First NameClaude Yasmine
Address81A Fourth Avenue Westdene
Emailclaudehamany@gmail.com
Phone0814415123
Submit Date2024-07-05
Num Groups4
Raw Data AvailableYes
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2024-09-03
Release Version1
Claude Yasmine Claude Yasmine Hamany Djande Claude Yasmine Claude Yasmine Hamany Djande
https://dx.doi.org/10.21228/M8HV52
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Plant; Subject species: Hordeum vulgare (Factor headings shown in green)

mb_sample_id local_sample_id Factor Sample source
SA371531120721ElimC12HC1bElimC12h Elim Control
SA371532120721ElimC12HA2bElimC12h Elim Control
SA371533120721ElimC12HC3bElimC12h Elim Control
SA371534120721ElimC12HC2bElimC12h Elim Control
SA371535120721ElimC12HA1bElimC12h Elim Control
SA371536120721ElimC12HB3bElimC12h Elim Control
SA371537120721ElimC12HB1bElimC12h Elim Control
SA371538120721ElimC12HA3bElimC12h Elim Control
SA371539120721ElimC12HB2bElimC12h Elim Control
SA371540120721ElimC24HA1bElimC24h Elim Control
SA371541120721ElimC24HA2bElimC24h Elim Control
SA371542120721ElimC24HA3bElimC24h Elim Control
SA371543120721ElimC24HB1bElimC24h Elim Control
SA371544120721ElimC24HB2bElimC24h Elim Control
SA371545120721ElimC24HB3bElimC24h Elim Control
SA371546120721ElimC24HC1bElimC24h Elim Control
SA371547120721ElimC24HC2bElimC24h Elim Control
SA371548120721ElimC24HC3bElimC24h Elim Control
SA371549120721ElimC36HC1bElimC36h Elim Control
SA371550120721ElimC36HC3bElimC36h Elim Control
SA371551120721ElimC36HC2bElimC36h Elim Control
SA371552120721ElimC36HA1bElimC36h Elim Control
SA371553120721ElimC36HB3bElimC36h Elim Control
SA371554120721ElimC36HB2bElimC36h Elim Control
SA371555120721ElimC36HB1bElimC36h Elim Control
SA371556120721ElimC36HA3bElimC36h Elim Control
SA371557120721ElimC36HA2bElimC36h Elim Control
SA371558120721ElimDCAA12HC3bElimDCAA12h Elim DCAA
SA371559120721ElimDCAA12HC1bElimDCAA12h Elim DCAA
SA371560120721ElimDCAA12HB3bElimDCAA12h Elim DCAA
SA371561120721ElimDCAA12HC2bElimDCAA12h Elim DCAA
SA371562120721ElimDCAA12HB1bElimDCAA12h Elim DCAA
SA371563120721ElimDCAA12HA3bElimDCAA12h Elim DCAA
SA371564120721ElimDCAA12HB2bElimDCAA12h Elim DCAA
SA371565120721ElimDCAA12HA2bElimDCAA12h Elim DCAA
SA371566120721ElimDCAA12HA1bElimDCAA12h Elim DCAA
SA371567120721ElimDCAA24HA2bElimDCAA24h Elim DCAA
SA371568120721ElimDCAA24HA3bElimDCAA24h Elim DCAA
SA371569120721ElimDCAA24HB1bElimDCAA24h Elim DCAA
SA371570120721ElimDCAA24HB2bElimDCAA24h Elim DCAA
SA371571120721ElimDCAA24HB3bElimDCAA24h Elim DCAA
SA371572120721ElimDCAA24HC1bElimDCAA24h Elim DCAA
SA371573120721ElimDCAA24HC2bElimDCAA24h Elim DCAA
SA371574120721ElimDCAA24HC3bElimDCAA24h Elim DCAA
SA371575120721ElimDCAA24HA1bElimDCAA24h Elim DCAA
SA371576120721ElimDCAA36HC1bElimDCAA36h Elim DCAA
SA371577120721ElimDCAA36HC3bElimDCAA36h Elim DCAA
SA371578120721ElimDCAA36HC2bElimDCAA36h Elim DCAA
SA371579120721ElimDCAA36HB1bElimDCAA36h Elim DCAA
SA371580120721ElimDCAA36HB3bElimDCAA36h Elim DCAA
SA371581120721ElimDCAA36HB2bElimDCAA36h Elim DCAA
SA371582120721ElimDCAA36HA2bElimDCAA36h Elim DCAA
SA371583120721ElimDCAA36HA1bElimDCAA36h Elim DCAA
SA371584120721ElimDCAA36HA3bElimDCAA36h Elim DCAA
SA371585120721QCAll1bQCAll QCAll
SA371586120721QCAll2bQCAll QCAll
SA371587120721QCAll3bQCAll QCAll
SA371588120721QCAll4bQCAll QCAll
SA371589120721QCAll5bQCAll QCAll
SA371590120721QCAll6bQCAll QCAll
SA371591120721QCAll7bQCAll QCAll
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