Summary of Study ST003898
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002439. The data can be accessed directly via it's Project DOI: 10.21228/M8CG2W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003898 |
| Study Title | Glycosaminoglycan-mediated lipoprotein uptake protects cancer cells from ferroptosis |
| Study Summary | Lipids are essential components of cancer cells due to their structural and signaling roles and many cancers take up extracellular lipids to meet metabolic demands. How these lipids contribute to cancer growth and progression remains poorly understood. Using functional genetic screens, lipoprotein uptake—the primary mechanism for lipid transport in circulation—is identify as a key determinant of ferroptosis sensitivity in cancer. Lipoprotein supplementation robustly inhibits ferroptosis across numerous cancer types, an effect largely driven by lipoprotein delivery of α-tocopherol. Mechanistically, cancer cells take up lipoproteins through a pathway dependent on sulfated glycosaminoglycans (GAGs) linked to cell-surface proteoglycans. Disrupting GAG biosynthesis or acutely degrading surface GAGs reduces lipoprotein uptake, sensitizes cancer cells to ferroptosis, and impairs tumor growth in mice. Notably, human clear cell renal cell carcinomas (ccRCC), a lipid-rich malignancy, exhibit elevated levels of chondroitin sulfate and increased lipoprotein-derived α-tocopherol compared to normal kidney tissue. Altogether, this work establishes lipoprotein uptake as a critical anti-ferroptotic mechanism in cancer and implicates GAG biosynthesis as a therapeutic target. |
| Institute | University of Texas Southwestern Medical Center at Dallas |
| Department | Children's Research Institute |
| Laboratory | Metabolomics Facility |
| Last Name | Cai |
| First Name | Feng |
| Address | 6000 Harry Hines Blvd. |
| feng.cai@utsouthwestern.edu | |
| Phone | 2146483056 |
| Submit Date | 2025-04-24 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-05-08 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample Information |
|---|---|---|
| SA429538 | 9456 (9) | VM100_Adjacent Kidney |
| SA429539 | 9456 (10) | VM100_Primary ccRCC |
| SA429540 | 9456 (11) | VM101_Adjacent Kidney |
| SA429541 | 9456 (12) | VM101_Primary ccRCC |
| SA429542 | 9456 (13) | VM102_Adjacent Kidney |
| SA429543 | 9456 (14) | VM102_Primary ccRCC |
| SA429544 | 9456 (15) | VM103_Adjacent Kidney |
| SA429545 | 9456 (16) | VM103_Primary ccRCC |
| SA429546 | 9456 (17) | VM104_Adjacent Kidney |
| SA429547 | 9456 (18) | VM104_Primary ccRCC |
| SA429548 | 9456 (19) | VM105_Adjacent Kidney |
| SA429549 | 9456 (20) | VM105_Primary ccRCC |
| SA429550 | 9456 (21) | VM106_Adjacent Kidney |
| SA429551 | 9456 (22) | VM106_Primary ccRCC |
| SA429552 | 9456 (23) | VM107_Adjacent Kidney |
| SA429553 | 9456 (24) | VM107_Primary ccRCC |
| SA429554 | 9456 (25) | VM108_Adjacent Kidney |
| SA429555 | 9456 (26) | VM108_Primary ccRCC |
| SA429556 | 9456 (27) | VM109_Adjacent Kidney |
| SA429557 | 9456 (28) | VM109_Primary ccRCC |
| SA429558 | 9456 (29) | VM110_Adjacent Kidney |
| SA429559 | 9456 (30) | VM110_Primary ccRCC |
| SA429560 | 9456 (31) | VM111_Adjacent Kidney |
| SA429561 | 9456 (32) | VM111_Primary ccRCC |
| SA429562 | 9456 (33) | VM112_Adjacent Kidney |
| SA429563 | 9456 (34) | VM112_Primary ccRCC |
| SA429564 | B4 (1) | VM91_Adjacent Kidney |
| SA429565 | B4 (2) | VM91_Primary ccRCC |
| SA429566 | B4 (3) | VM92_Adjacent Kidney |
| SA429567 | B4 (4) | VM92_Primary ccRCC |
| SA429568 | B4 (5) | VM94_Adjacent Kidney |
| SA429569 | B4 (6) | VM94_Primary ccRCC |
| SA429570 | 9456 (1) | VM95_Adjacent Kidney |
| SA429571 | 9456 (2) | VM95_Primary ccRCC |
| SA429572 | 9456 (3) | VM96_Adjacent Kidney |
| SA429573 | 9456 (4) | VM96_Primary ccRCC |
| SA429574 | 9456 (5) | VM97_Adjacent Kidney |
| SA429575 | 9456 (6) | VM97_Primary ccRCC |
| SA429576 | 9456 (7) | VM98_Adjacent Kidney |
| SA429577 | 9456 (8) | VM98_Primary ccRCC |
| Showing results 1 to 40 of 40 |
