Summary of Study ST000074

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000071. The data can be accessed directly via it's Project DOI: 10.21228/M8G01M This work is supported by NIH grant, U2C- DK119886.

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Study IDST000074
Study TitleGenetic effects of high fat diet on mouse fecal metabolomics
Study SummaryThis study includes 72 female mice with 4 mice from each of the 18 mice strains. Two mice from each strain were fed a high fat diet and two mice were fed a normal fat diet. The 36 mice fed a normal fat diet will serve as the controls. All mice are age 27.6 weeks or older at the time of sacrifice. UPLC-MS data was collected for all 72 samples.
Institute
University of North Carolina
DepartmentDiscovery and Analytical Sciences (DAS)
LaboratorySumner Lab
Last NameSumner
First NameSusan
AddressEastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Emailsusan_sumner @unc.edu
Phone704-250-5066
Submit Date2014-06-14
Num Groups36
Total Subjects72
Raw Data AvailableNo
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2015-06-01
Release Version1
Susan Sumner Susan Sumner
https://dx.doi.org/10.21228/M8G01M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000117 AN000118
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Waters Acquity Waters Acquity
Column Waters Acquity HSS T3 (100 x 2.1mm,1.8um) Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Waters Synapt G2 QTOF Waters Synapt G2 QTOF
Ion Mode POSITIVE NEGATIVE
Units Normalized abundance Normalized abundance

MS:

MS ID:MS000093
Analysis ID:AN000117
Instrument Name:Waters Synapt G2 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Capillary Temperature:110 C
Capillary Voltage:3.2 kV
Collision Energy:4
Helium Flow:180
Ionization:ES+
Mass Accuracy:10 ppm
Source Temperature:110 C
Dataformat:Continuum
Desolvation Gas Flow:400 L/Hr
Desolvation Temperature:400 C
Resolution Setting:18000
Scan Range Moverz:50-1000 m/s
Scanning Cycle:1 s
Tube Lens Voltage:75
  
MS ID:MS000094
Analysis ID:AN000118
Instrument Name:Waters Synapt G2 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Capillary Temperature:110 C
Capillary Voltage:2.8 kV
Collision Energy:4
Helium Flow:180
Ionization:ES-
Source Temperature:110 C
Dataformat:Continuum
Desolvation Gas Flow:400 L/Hr
Desolvation Temperature:400 C
Resolution Setting:18000
Scan Range Moverz:50-1000 m/z
Scanning Cycle:1 s
Tube Lens Voltage:74
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