Summary of Study ST000247
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000199. The data can be accessed directly via it's Project DOI: 10.21228/M8P304 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000247 |
| Study Title | Determining the metabolic profile of wildtype and nos mutant Staphylococcus aureus grown in media lacking glucose using targeted LC/MS |
| Study Type | Single time point |
| Study Summary | Whole cells from wildtype, nos mutant, SrrAB mutant, SrrAB nos double mutant, and complement strains will be isolated for targeted metabolite analysis. In parallel, supernatants (extracellular metabolites) will also be analyzed for their metabolic profile. |
| Institute | University of Florida |
| Department | SECIM |
| Last Name | Rice |
| First Name | Kelly |
| kcrice@ufl.edu | |
| Submit Date | 2015-02-17 |
| Num Groups | 7 |
| Total Subjects | 21 |
| Raw Data File Type(s) | raw(Thermo), d |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-01-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000387 | AN000388 | AN000389 | AN000390 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Agilent 1290 Infinity |
| Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) | Thermo Hypercarb (50 x 2.1mm,3um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) | Waters AccQ Tag (100 x 2mm,1.7um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole |
| MS instrument name | Thermo Quantiva QQQ | Thermo Quantiva QQQ | Thermo Quantiva QQQ | Agilent 6490 QQQ |
| Ion Mode | POSITIVE | POSITIVE | POSITIVE | POSITIVE |
| Units | nmol/mg protein | nmol/mg protein | nmol/mg protein | nmol/mg protein |
MS:
| MS ID: | MS000331 |
| Analysis ID: | AN000387 |
| Instrument Name: | Thermo Quantiva QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Organic Acids (Instrument:Thermo Scientific Quantiva) |
| Ion Mode: | POSITIVE |
| MS ID: | MS000332 |
| Analysis ID: | AN000388 |
| Instrument Name: | Thermo Quantiva QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | NAD(Instrument:Thermo Scientific Quantiva) |
| Ion Mode: | POSITIVE |
| MS ID: | MS000333 |
| Analysis ID: | AN000389 |
| Instrument Name: | Thermo Quantiva QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | NADH(Instrument:Thermo Scientific Quantiva) |
| Ion Mode: | POSITIVE |
| MS ID: | MS000334 |
| Analysis ID: | AN000390 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Amino Acids(Instrument:Agilent 6490) |
| Ion Mode: | POSITIVE |
