Summary of Study ST000282

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000224. The data can be accessed directly via it's Project DOI: 10.21228/M8K599 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000282
Study TitlePilot Study 13C flux effects when RhoC or RhoA perturbed (13C BCs)
Study Type13C mass isotopomer analysis (flux studies)
Study SummaryRho-GTPases are small GTP-binding proteins that contribute to the epithelial-to-mesenchymal transition by regulating several cellular processes including organization of the actin cytoskeleton, cell motility, transcription, and cell proliferation. Overexpression of RhoC-GTPases (RhoC) in breast cancer has been implicated in poor disease prognosis due to increased cancer cells invasion, migration, and motility, which warranted its consideration as a therapeutic target for inhibiting breast cancer metastasis. Using silencing RNA (siRNA) molecules to knockdown RhoC expression is a promising approach to inhibit breast cancer metastases.
Institute
University of Michigan
DepartmentInternal Medicine
LaboratoryMerajver Lab
Last NameWynn
First NameMichelle
AddressUniversity Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105
Emailmlwynn@umich.edu
Phone734-647-3408
Submit Date2015-02-11
Num Groups5
Total Subjects16
Raw Data File Type(s)d
Analysis Type DetailGC/LC-MS
Release Date2016-01-13
Release Version1
Michelle Wynn Michelle Wynn
https://dx.doi.org/10.21228/M8K599
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000449 AN000450
Analysis type MS MS
Chromatography type HILIC GC
Chromatography system Agilent 1260 Agilent 7890A
Column Phenomenex Luna NH2 (150 x 1mm, 3um) Agilent DB5-MS (30m × 0.25mm, 0.25um)
MS Type ESI EI
MS instrument type QTOF Single quadrupole
MS instrument name Agilent 6530 QTOF Agilent 5975C
Ion Mode NEGATIVE POSITIVE
Units Area Area normalized

MS:

MS ID:MS000390
Analysis ID:AN000449
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Acquisition Parameters File:QTOF-002-HILIC-35min-1mm.m.zip
Processing Parameters File:EX00403-flux-MassHunterQuant-GlyTCA-DataAnalysis-LCMS-Method.m.zip
  
MS ID:MS000391
Analysis ID:AN000450
Instrument Name:Agilent 5975C
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
Acquisition Parameters File:MAHMOUD-PALM_14ISOTOPE.M.zip
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