Summary of Study ST000828

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000219. The data can be accessed directly via it's Project DOI: 10.21228/M8359Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST000828
Study TitleMetabolic analysis of Parp1 ko/wt Saline & Bleo Mouse Lung Fiboblasts and Human IPF & Normal Lung Fiboblasts 3
Study TypeMS analysis
Study SummaryGlycolysis/TCA/Nucleotide analysis (especially interested in alpha-ketoglutarate) and NAD+ and related metabolite analysis for all samples
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-08-02
Num Groups6
Total Subjects16
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC-MS/LC-MS
Release Date2017-10-11
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8359Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN001834 AN001835 AN001836 AN001837
Analysis type MS MS MS MS
Chromatography type Phenyl Phenyl HILIC GC
Chromatography system Agilent Agilent Agilent Agilent GC_7890N
Column Restek Pinnacle DB Biphenyl (50 x 2.1mm,1.9um,140A) Restek Pinnacle DB Biphenyl (50 x 2.1mm,1.9um,140A) Phenomenex Luna NH2 (150 x 1mm,3um) Agilent DB5-MS (30m × 0.25mm, 0.25um)
MS Type ESI ESI ESI EI
MS instrument type Triple quadrupole Triple quadrupole QTOF Single quadrupole
MS instrument name Agilent 6490A QQQ Agilent 6490A QQQ Agilent 6520B QTOF Agilent 5975
Ion Mode POSITIVE POSITIVE NEGATIVE POSITIVE
Units counts uM uM uM

MS:

MS ID:MS001695
Analysis ID:AN001834
Instrument Name:Agilent 6490A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:none
Ion Mode:POSITIVE
Acquisition Parameters File:QM033_2mmC18_Ceramide_MRM
Analysis Protocol File:A010_Ceramides.pdf
  
MS ID:MS001696
Analysis ID:AN001835
Instrument Name:Agilent 6490A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:none
Ion Mode:POSITIVE
Acquisition Parameters File:QM033_2mmC18_Ceramide_MRM
Analysis Protocol File:A010_Ceramides.pdf
  
MS ID:MS001697
Analysis ID:AN001836
Instrument Name:Agilent 6520B QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:none
Ion Mode:NEGATIVE
Acquisition Parameters File:QTOF-002-HILIC-35min-1mm.m
Analysis Protocol File:A011_Glycolysis-TCA-nucleotides.pdf
  
MS ID:MS001698
Analysis ID:AN001837
Instrument Name:Agilent 5975
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:none
Ion Mode:POSITIVE
Acquisition Parameters File:ALPHA KETO ACIDS-FULL.M
Analysis Protocol File:A011_Glycolysis-TCA-nucleotides.pdf
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