Summary of Study ST001143

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000764. The data can be accessed directly via it's Project DOI: 10.21228/M8XH5B This work is supported by NIH grant, U2C- DK119886.

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Study IDST001143
Study TitleMicrobial depletion and ozone exposure - Lung tissue (part I)
Study SummaryGlobal biochemical profiles were determined in lung tissue collected from untreated control mice and mice treated for two weeks with untreated drinking water or water containing an antibiotic cocktail (ampicillin, neomycin, metronidazole, and vancomycin), followed by a three hour exposure to ambient air or ozone (2ppm). Sample collection occurred 24 hours post-ozone exposure.
Institute
Harvard School of Public Health
Last NameShore
First NameStephanie
Address677 Huntington Ave
Emailsshore@hsph.harvard.edu
Phone6174320989
Submit Date2019-02-26
Raw Data AvailableYes
Analysis Type DetailGC-MS/LC-MS
Release Date2019-05-15
Release Version1
Stephanie Shore Stephanie Shore
https://dx.doi.org/10.21228/M8XH5B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN001882 AN001883 AN001884 AN001885
Analysis type MS MS MS MS
Chromatography type Reversed phase/HILIC Reversed phase/HILIC Reversed phase/HILIC GC
Chromatography system Waters Acquity Waters Acquity Waters Acquity Thermo Trace DSQ
Column Waters Acquity BEH C18 (100 x 2mm,1.7um)/Waters UPLC BEH Amide (2.1x150 mm,1.7um) Waters Acquity BEH C18 (100 x 2mm,1.7um)/Waters UPLC BEH Amide (2.1x150 mm,1.7um) Waters Acquity BEH C18 (100 x 2mm,1.7um)/Waters UPLC BEH Amide (2.1x150 mm,1.7um) 5% diphenyl/95% dimethyl polysiloxane GC column
MS Type ESI ESI ESI EI
MS instrument type Orbitrap Orbitrap Orbitrap Single quadrupole
MS instrument name Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap Thermo Trace DSQ
Ion Mode POSITIVE NEGATIVE NEGATIVE POSITIVE
Units area under the curve area under the curve area under the curve area under the curve

MS:

MS ID:MS001738
Analysis ID:AN001882
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Reversed phase LC-MS Positive
Ion Mode:POSITIVE
  
MS ID:MS001739
Analysis ID:AN001883
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Reversed phase LC-MS Negative
Ion Mode:NEGATIVE
  
MS ID:MS001740
Analysis ID:AN001884
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:HILIC LC-MS Negative
Ion Mode:NEGATIVE
  
MS ID:MS001741
Analysis ID:AN001885
Instrument Name:Thermo Trace DSQ
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:GC-MS
Ion Mode:POSITIVE
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