Summary of Study ST001398

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000958. The data can be accessed directly via it's Project DOI: 10.21228/M8VM30 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001398
Study TitleMechanism of Trichloroethylene (TCE) toxicity in the placenta
Study TypeMS analysis
Study SummaryCells in culture will be exposed to S-(1,2-dichlorovinyl)-L-cysteine (DCVC), a relevant metabolite of TCE. The first cell line used will be HTR-8/SVneo, originally derived from first trimester female human cytotrophoblast cells and immortalized with simian virus 40 large T antigen, this cell line models first-trimester placental extravillous trophoblasts in vitro. HTR-8/SVneo cells will be cultured for 24 hours followed by exposure to cell culture media (control) or 20 ┬ÁM DCVC for 6 or 12 hours. Cells (cells frozen in cell culture dish) will then be collected (n=5) In the TCE aim of this study, we will utilize one cell model that phenotypically represents an important placental cell population.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
AddressAnn Arbor, MI
Emailmkachman@med.umich.edu
Phone734-232-0842
Submit Date2020-06-08
Publicationshttps://pubmed.ncbi.nlm.nih.gov/31951115/
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2020-06-18
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8VM30
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002337 AN002338
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Agilent 1200 Agilent 1200
Column Phenomenex Luna NH2 (150 x 2.1mm, 3um) Phenomenex Luna NH2 (150 x 2.1mm, 3um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6520 QTOF Agilent 6520 QTOF
Ion Mode NEGATIVE NEGATIVE
Units area/ug protein pmol/ug

MS:

MS ID:MS002179
Analysis ID:AN002337
Instrument Name:Agilent 6520 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Quantitative data analysis using Agilent Quant software
Ion Mode:NEGATIVE
  
MS ID:MS002180
Analysis ID:AN002338
Instrument Name:Agilent 6520 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Semi-quantitative data analysis using Agilent Quant software
Ion Mode:NEGATIVE
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