Summary of study ST001859

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001172. The data can be accessed directly via it's Project DOI: 10.21228/M86T2S This work is supported by NIH grant, U2C- DK119886.

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Study IDST001859
Study TitledTor affects the fat body lipidome via Nep1r1, Ctdnep1 and Lipin
Study SummaryQuantitative MS analysis was performed on ten 4 day-old Drosophila larval fat bodies homogenized in 50µl D-PBS (Dulbecco’s Phosphate Buffered Saline without Mg2+ and Ca2+) by Lipotype using previously described methods (Grillet et al, 2016).
Institute
VIB KULeuven
DepartmentDept. of Neurosciences, KU Leuven, 3000 Leuven, Belgium
Last NameJacquemyn
First NameJulie
AddressON 4, 6e verd Campus Gasthuisberg, Herestraat 49, bus 602, Leuven, NA, 3000, Belgium
Emailjulie.jacquemyn@kuleuven.be
Phone0032479570951
Submit Date2021-06-22
Analysis Type DetailLC-MS
Release Date2021-07-18
Release Version1
Julie Jacquemyn Julie Jacquemyn
https://dx.doi.org/10.21228/M86T2S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003013 AN003014
Analysis type MS MS
Chromatography type None (Direct infusion) None (Direct infusion)
Chromatography system none none
Column none none
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units pmol pmol

MS:

MS ID:MS002802
Analysis ID:AN003013
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Spectra acquisition Mass spectra were acquired on a hybrid quadrupole/Orbitrap mass spectrometer equipped with an automated nano-flow electrospray ion source in both positive and negative ion mode. Data processing and normalization Lipid identification using LipotypeXplorer (2) was performed on unprocessed (*.raw format) mass spectra. For MS-only mode, lipid identification was based on the molecular masses of the intact molecules. MSMS mode included the collision induced fragmentation of lipid molecules and lipid identification was based on both the intact masses and the masses of the fragments. Prior to normalization and further statistical analysis lipid identifications were filtered according to mass accuracy, occupation threshold, noise and background. Lists of identified lipids and their intensities were stored in a database optimized for the particular structure inherent to lipidomic datasets. Intensity of lipid class-specific internal standards was used for lipid quantification. (2) Herzog, R. et al. A novel informatics concept for high-throughput shotgun lipidomics based on the molecular fragmentation query language. Genome Biol. 12, R8 (2011).
Ion Mode:POSITIVE
  
MS ID:MS002803
Analysis ID:AN003014
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Spectra acquisition Mass spectra were acquired on a hybrid quadrupole/Orbitrap mass spectrometer equipped with an automated nano-flow electrospray ion source in both positive and negative ion mode. Data processing and normalization Lipid identification using LipotypeXplorer (2) was performed on unprocessed (*.raw format) mass spectra. For MS-only mode, lipid identification was based on the molecular masses of the intact molecules. MSMS mode included the collision induced fragmentation of lipid molecules and lipid identification was based on both the intact masses and the masses of the fragments. Prior to normalization and further statistical analysis lipid identifications were filtered according to mass accuracy, occupation threshold, noise and background. Lists of identified lipids and their intensities were stored in a database optimized for the particular structure inherent to lipidomic datasets. Intensity of lipid class-specific internal standards was used for lipid quantification. (2) Herzog, R. et al. A novel informatics concept for high-throughput shotgun lipidomics based on the molecular fragmentation query language. Genome Biol. 12, R8 (2011).
Ion Mode:NEGATIVE
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