Summary of Study ST002930

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001822. The data can be accessed directly via it's Project DOI: 10.21228/M86H8Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002930
Study TitleRole of Hypoxia-inducible factor-1a (HIF1a)in Skeletal Muscle Physiology (C2C12 myotube model)
Study SummaryHypoxia-inducible factor (HIF)-1a is continuously synthesized and degraded in normoxia, whereas during hypoxia, HIF1a stabilization restricts cellular oxygen utilization. Less is known about HIF1a function(s) and sex-specific effects during normoxia in the basal state. Since skeletal muscle is the largest protein store in mammals and protein homeostasis has high energy demands, we determined HIF1a function at baseline during normoxia in C2C12 murine myotubes with the use of untargeted metabolomics. 114 samples of extracted metabolites from cells were analyzed using LCMS. We identified that metabolites, especially those in the glycolytic pathway and TCA cycle, were differentially expressed in cells with HIF1a KO compared to WT.
Institute
Cleveland Clinic
DepartmentInflamation and Immunity
LaboratoryDasarathy Lab
Last NameDasarathy
First NameSrinivasan
Address9500 Euclid Avenue
Emaildasaras@ccf.org
Phone2163799846
Submit Date2023-10-05
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2024-10-28
Release Version1
Srinivasan Dasarathy Srinivasan Dasarathy
https://dx.doi.org/10.21228/M86H8Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN004807 AN004808
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Thermo Vanquish Thermo Vanquish
Column Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type QTRAP QTRAP
MS instrument name Thermo Q Exactive Focus Thermo Q Exactive Focus
Ion Mode POSITIVE NEGATIVE
Units relative abundance relative abundance

MS:

MS ID:MS004553
Analysis ID:AN004807
Instrument Name:Thermo Q Exactive Focus
Instrument Type:QTRAP
MS Type:ESI
MS Comments:Data dependent acquisitions (DDA) on the pooled representative QC samples include MS full scans at a resolution of 120,000 and HCD MS/MS scans taken on the top 5 most abundant ions at a resolution of 30,000 with dynamic exclusion. The resolution of the MS2 scans were taken at a stepped NCE energy of 10.0, 20.0 and 30.0.
Ion Mode:POSITIVE
  
MS ID:MS004554
Analysis ID:AN004808
Instrument Name:Thermo Q Exactive Focus
Instrument Type:QTRAP
MS Type:ESI
MS Comments:Data dependent acquisitions (DDA) on the pooled representative QC samples include MS full scans at a resolution of 120,000 and HCD MS/MS scans taken on the top 5 most abundant ions at a resolution of 30,000 with dynamic exclusion. The resolution of the MS2 scans were taken at a stepped NCE energy of 10.0, 20.0 and 30.0.
Ion Mode:NEGATIVE
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