Summary of Study ST002930
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001822. The data can be accessed directly via it's Project DOI: 10.21228/M86H8Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002930 |
Study Title | Role of Hypoxia-inducible factor-1a (HIF1a)in Skeletal Muscle Physiology (C2C12 myotube model) |
Study Summary | Hypoxia-inducible factor (HIF)-1a is continuously synthesized and degraded in normoxia, whereas during hypoxia, HIF1a stabilization restricts cellular oxygen utilization. Less is known about HIF1a function(s) and sex-specific effects during normoxia in the basal state. Since skeletal muscle is the largest protein store in mammals and protein homeostasis has high energy demands, we determined HIF1a function at baseline during normoxia in C2C12 murine myotubes with the use of untargeted metabolomics. 114 samples of extracted metabolites from cells were analyzed using LCMS. We identified that metabolites, especially those in the glycolytic pathway and TCA cycle, were differentially expressed in cells with HIF1a KO compared to WT. |
Institute | Cleveland Clinic |
Department | Inflamation and Immunity |
Laboratory | Dasarathy Lab |
Last Name | Dasarathy |
First Name | Srinivasan |
Address | 9500 Euclid Avenue |
dasaras@ccf.org | |
Phone | 2163799846 |
Submit Date | 2023-10-05 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2024-10-28 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004807 | AN004808 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Thermo Vanquish | Thermo Vanquish |
Column | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | QTRAP | QTRAP |
MS instrument name | Thermo Q Exactive Focus | Thermo Q Exactive Focus |
Ion Mode | POSITIVE | NEGATIVE |
Units | relative abundance | relative abundance |
MS:
MS ID: | MS004553 |
Analysis ID: | AN004807 |
Instrument Name: | Thermo Q Exactive Focus |
Instrument Type: | QTRAP |
MS Type: | ESI |
MS Comments: | Data dependent acquisitions (DDA) on the pooled representative QC samples include MS full scans at a resolution of 120,000 and HCD MS/MS scans taken on the top 5 most abundant ions at a resolution of 30,000 with dynamic exclusion. The resolution of the MS2 scans were taken at a stepped NCE energy of 10.0, 20.0 and 30.0. |
Ion Mode: | POSITIVE |
MS ID: | MS004554 |
Analysis ID: | AN004808 |
Instrument Name: | Thermo Q Exactive Focus |
Instrument Type: | QTRAP |
MS Type: | ESI |
MS Comments: | Data dependent acquisitions (DDA) on the pooled representative QC samples include MS full scans at a resolution of 120,000 and HCD MS/MS scans taken on the top 5 most abundant ions at a resolution of 30,000 with dynamic exclusion. The resolution of the MS2 scans were taken at a stepped NCE energy of 10.0, 20.0 and 30.0. |
Ion Mode: | NEGATIVE |