Summary of Study ST003553

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002188. The data can be accessed directly via it's Project DOI: 10.21228/M8SV5J This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003553
Study TitleInvestigation of age-dependent changes in the gut of honeybee workers using targeted metabolomics for amino acid and biogenic amine determination
Study SummaryIn the honeybee guts we measured the concentrations of 20 amino acids and 6 biogenic amines. Tryptophan and its downstream metabolite kynurenine followed the same trends across the worker development, with a significant decrease in their concentrations from day 3 onwards. Serotonin, on the other hand, was significantly increased from day 1. Tyramine and octopamine showed significant increases in concentrations (octopamine for all age groups apart from Day 1; tyramine for all age groups), while their precursor tyrosine showed significantly decreased concentrations for all age groups except experienced foragers.
Institute
Helmholtz Centre for Environmental Research
DepartmentMolecular Systems Biology
Last NameEngelmann
First NameBeatrice
AddressPermoserstraße 15, Leipzipg, Saxony, 03418, Germany
Emailbeatrice.engelmann@ufz.de
Phone004934160251099
Submit Date2024-10-28
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2024-11-27
Release Version1
Beatrice Engelmann Beatrice Engelmann
https://dx.doi.org/10.21228/M8SV5J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN005840 AN005841
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Waters Acquity Waters Acquity
Column Zorbax Eclipse XDB-C18 (100 x 3.0mm, 3.5um) Zorbax Eclipse XDB-C18 (100 x 3.0mm, 3.5um)
MS Type ESI ESI
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name ABI Sciex 5500 QTrap ABI Sciex 6500+ Qtrap
Ion Mode POSITIVE POSITIVE
Units µMol µMol

MS:

MS ID:MS005560
Analysis ID:AN005840
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:For identification and quantitation, a scheduled MRM method was used, with specific transitions for every amino acid and biogenic amine. Data acquisition and peak integration were performed in SciexOS software (Version 3.0.0.). Calculation of concentration was done using external calibration curves.
Ion Mode:POSITIVE
  
MS ID:MS005561
Analysis ID:AN005841
Instrument Name:ABI Sciex 6500+ Qtrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:For identification and quantitation, a scheduled MRM method was used, with specific transitions for every amino acid and biogenic amine. Data acquisition and peak integration were performed in SciexOS software (Version 3.0.0.). Calculation of concentration was done using external calibration curves.
Ion Mode:POSITIVE
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