Summary of Study ST000049
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000024. The data can be accessed directly via it's Project DOI: 10.21228/M85P4V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000049 |
| Study Title | Metabolomics Analysis of Thermally Challenged Mayfly Larvae (NMR analysis) |
| Study Type | Metabolomic analysis of mayflies |
| Study Summary | The purpose of this study was to examine the metabolic profiles of mayfly (Centroptilum triangulifer) larvae subjected to thermal challenge. This species is unusual in terms of its ease of culture, and its suitability as a laboratory test organism. Our purpose here was to examine how an environmentally realistic thermal challenge affects the physiology of this organism. In this study, we obtained several types of insect species and we were able to show that NMR Metabolomics could be used to distinguish among the different types of larvae. |
| Institute | University of North Carolina |
| Department | Discovery Science Technology |
| Laboratory | Sumner Lab |
| Last Name | Sumner |
| First Name | Susan |
| Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
| susan_sumner @unc.edu | |
| Phone | 704-250-5066 |
| Submit Date | 2014-02-28 |
| Num Groups | 7 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Uploaded File Size | 7.6 M |
| Analysis Type Detail | NMR |
| Release Date | 2015-05-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
NMR:
| NMR ID: | NM000022 |
| Analysis ID: | AN000085 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Field Frequency Lock: | D20 |
| Standard Concentration: | 0.5 mM |
| Spectrometer Frequency: | 950 MHz |
| NMR Probe: | 5mm Cryogenically Cooled ATMA |
| NMR Solvent: | D20 |
| NMR Tube Size: | 5mm |
| Shimming Method: | Topshim (Gradient) |
| Pulse Sequence: | noesypr1d |
| Water Suppression: | yes |
| Pulse Width: | 8.68 |
| Power Level: | 12.589W |
| Receiver Gain: | 4 |
| Offset Frequency: | 4468.30 Hz |
| Chemical Shift Ref Cpd: | DSS |
| Temperature: | 298 K |
| Number Of Scans: | 256 |
| Dummy Scans: | 4 |
| Acquisition Time: | 1.73 sec |
| Relaxation Delay: | 2s |
| Spectral Width: | 18939.395 Hz |
| Num Data Points Acquired: | 65536 |
| Real Data Points: | 65536 |
| Line Broadening: | 0.5 |
| Zero Filling: | Yes |
| Apodization: | Lorentzian |
| Baseline Correction Method: | Polynomial |
| Chemical Shift Ref Std: | DSS |
