Summary of Study ST000134
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000119. The data can be accessed directly via it's Project DOI: 10.21228/M86C76 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000134 |
| Study Title | Monitoring In Vitro Response of Selenium-Treated Prostate Cells by 1H NMR Spectroscopy |
| Study Type | Metabolite level response after treatment with organoselenium |
| Study Summary | Metabolomics analysis was performed on DU145 prostate cancer cells and PNT1A non-tumorigenic prostate cells after treatment with selenomethionine and Se-methylselenocysteine using 800 MHz Bruker NMR spectrometer on 18 cell samples. |
| Institute | Purdue University |
| Department | Biology/Chemistry |
| Laboratory | Biology/Chemistry |
| Last Name | Isaac-Lam |
| First Name | Meden |
| Address | 1401 S US Hwy 421, Westville, IN 46391 |
| isaaclam@pnc.edu | |
| Phone | 219-785-5776 |
| Submit Date | 2015-01-13 |
| Num Groups | 6 |
| Total Subjects | 18 |
| Raw Data Available | No |
| Analysis Type Detail | NMR |
| Release Date | 2015-01-13 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
NMR:
| NMR ID: | NM000050 |
| Analysis ID: | AN000216 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Field Frequency Lock: | Deuterium |
| Standard Concentration: | 1.43 mM |
| Spectrometer Frequency: | 800 MHz |
| NMR Probe: | TX1, inverse |
| NMR Solvent: | D2O |
| NMR Tube Size: | 5 mm x 7 in |
| Shimming Method: | gradient, topshim |
| Pulse Sequence: | zg one-pulse |
| Water Suppression: | presaturation |
| Pulse Width: | 8.5 msec |
| Power Level: | 12.25 W |
| Receiver Gain: | 912 |
| Offset Frequency: | 4.8 ppm |
| Presaturation Power Level: | 57 dB |
| Chemical Shift Ref Cpd: | TMSP |
| Temperature: | 295.9 |
| Number Of Scans: | 256 |
| Dummy Scans: | 8 |
| Acquisition Time: | 1.4680564 |
| Relaxation Delay: | 6.5 msec |
| Spectral Width: | 11160 Hz |
| Num Data Points Acquired: | 32 K |
| Real Data Points: | 32 K |
| Line Broadening: | 1 Hz |
| Zero Filling: | 16K |
| Apodization: | lorentzian |
| Baseline Correction Method: | polynomial |
| Chemical Shift Ref Std: | TMSP |
