Summary of Study ST000134
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000119. The data can be accessed directly via it's Project DOI: 10.21228/M86C76 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000134 |
| Study Title | Monitoring In Vitro Response of Selenium-Treated Prostate Cells by 1H NMR Spectroscopy |
| Study Type | Metabolite level response after treatment with organoselenium |
| Study Summary | Metabolomics analysis was performed on DU145 prostate cancer cells and PNT1A non-tumorigenic prostate cells after treatment with selenomethionine and Se-methylselenocysteine using 800 MHz Bruker NMR spectrometer on 18 cell samples. |
| Institute | Purdue University |
| Department | Biology/Chemistry |
| Laboratory | Biology/Chemistry |
| Last Name | Isaac-Lam |
| First Name | Meden |
| Address | 1401 S US Hwy 421, Westville, IN 46391 |
| isaaclam@pnc.edu | |
| Phone | 219-785-5776 |
| Submit Date | 2015-01-13 |
| Num Groups | 6 |
| Total Subjects | 18 |
| Raw Data Available | No |
| Analysis Type Detail | NMR |
| Release Date | 2015-01-13 |
| Release Version | 1 |
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Project:
| Project ID: | PR000119 |
| Project DOI: | doi: 10.21228/M86C76 |
| Project Title: | Monitoring In Vitro Response of Selenium-Treated Prostate Cells by 1H NMR Spectroscopy |
| Project Type: | Metabolomics by 1H NMR |
| Project Summary: | 1H NMR data of prostate cells treated with selenium showed a decreasing trend in metabolite levels with the largest change exhibited by creatine mainly due to disrupted energy metabolism, and probably due to loss of structural integrity combined with external dissipation of metabolites. Lactate, choline-containing compounds, and glycine levels increased depending on the type of selenium used and the cell type. Principal component analysis (PCA) showed that SeM-treated cells can be distinguished from SeMSC-treated cells, and DU145 PCa from PNT1A normal cells. |
| Institute: | Purdue University |
| Department: | Biology/Chemistry |
| Laboratory: | Biology/Chemistry Dept |
| Last Name: | Isaac-Lam |
| First Name: | Meden |
| Address: | 1401 S US Hwy 421 Westville, Indiana USA |
| Email: | isaaclam@pnc.edu |
| Phone: | 1-219-785-5776 |
| Funding Source: | Indiana Academy of Science |
