Summary of Study ST000140
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000125. The data can be accessed directly via it's Project DOI: 10.21228/M8DW2B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000140 |
| Study Title | Cell Rinsing Solution Comparison |
| Study Type | Cell Rinsing Solution Comparison |
| Study Summary | Aliquots of Jurkat T-lymphocyte cells were washed with 5 different rinsing solutions (0.3% amm. formate, 0.3% amm. acetate, 0.9% NaCl, 1 M PBS, 100 mM PBS) and ran in triplicate to monitor the effect of ion suppression on the electrospray ionization signal. |
| Institute | University of Florida |
| Department | Dept. of Chemistry/SECIM |
| Laboratory | Biomedical Mass Spectrometry Lab |
| Last Name | Ulmer |
| First Name | Candice |
| Address | P.O. Box 117200, Gainesville, FL 32611 |
| czulmer@chem.ufl.edu | |
| Phone | (352) 392-0515 |
| Submit Date | 2015-03-02 |
| Num Groups | 5 |
| Total Subjects | 15 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Uploaded File Size | 3.1 G |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-03-03 |
| Release Version | 1 |
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Project:
| Project ID: | PR000125 |
| Project DOI: | doi: 10.21228/M8DW2B |
| Project Title: | Mammalian Suspension-Cultured Cellular Metabolomics Workflow |
| Project Type: | Metabolomics, Lipidomics, Untargeted |
| Project Summary: | A workflow was optimized for the sample preparation of a single suspension-cultured cell pellet for both metabolomics and lipidomics analysis. Jurkat T-lymphocyte cells were washed with various rinsing solutions and the lipids extracted using different lipid extraction protocols to allow for the most reproducible and quantitative method. |
| Institute: | University of Florida |
| Department: | Dept. of Chemistry/SECIM |
| Laboratory: | Biomedical Mass Spectrometry Lab |
| Last Name: | Yost |
| First Name: | Richard |
| Address: | P.O. Box 117200, Gainesville, FL 32611 |
| Email: | ryost@aa.ufl.edu |
| Phone: | (352) 392-0515 |
| Funding Source: | JDRF Research Grant |
