Summary of Study ST000253
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000205. The data can be accessed directly via it's Project DOI: 10.21228/M8WK5Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000253 |
| Study Title | NIH WCMC Pilot & Feasibility Project: “Metabolomics of Neonatal Pulmonary Hypertension” |
| Study Type | Treatment and feeding study |
| Study Summary | Targeted metabolomic analyses of oxylipins were performed on 16 rat plasma and lung samples collected from rats euthanized at 14 days following exposure to growth restriction and/or hyperoxia. Samples were analyzed by UPLC-MS/MS using a Waters Acquity UPLC and detected on an API 4000 QTrap (AB Sciex, Framingham, MA, USA) by multiple reaction monitoring (MRM) after negative mode electrospray ionization. |
| Institute | University of California, Davis |
| Department | U.S.D.A. Western Human Nutrition Research Center |
| Laboratory | Newman Lab |
| Last Name | Newman |
| First Name | John |
| Address | 430 W. Health Sciences Dr., Davis, CA 95616 |
| john.newman@ars.usda.gov | |
| Phone | +1-530-752-1009 |
| Submit Date | 2015-09-03 |
| Num Groups | 4 |
| Total Subjects | 16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Uploaded File Size | 16 M |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-09-14 |
| Release Version | 1 |
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Project:
| Project ID: | PR000205 |
| Project DOI: | doi: 10.21228/M8WK5Q |
| Project Title: | Metabolomics of Neonatal Pulmonary Hypertension (in a Rat Model) |
| Project Type: | Treatment and feeding study |
| Project Summary: | This study aims to identify identify disrupted metabolic pathways and patterns of lung developent in a rat model of bronchopulmonary dysplasia and growth restriction. Pregnant Sprague Dawley rats (gestational day 15) were housed in plsatic cages under constant conditions. After 7-d acclimization period, litters were delivered and pups randomly distributed between litters of 10 pups (N, normal intake) or litters of 17 pups (R, restricted intake). Normal and restriced intake pups were exposed to 75% oxygen (O, oxygen) in a Plexiglass chamber or to 21% oxygen (A, room air) continuously for 14 days, with daily rotation of dams to avoid oxygen toxicity. Both plasma and lung tissue underwent metabolomics analysis. |
| Institute: | University of California, Davis |
| Department: | Department of Pediatrics, School of Medicine |
| Laboratory: | Wedgwood lab |
| Last Name: | Wedgwood |
| First Name: | Stephen |
| Address: | 1002B, Research II, 4625 2nd Ave., Sacramento, CA 95817 |
| Email: | stephen.wedwood@ucdmc.ucdavis.edu |
| Phone: | +1-916-734-1518 |
| Funding Source: | National Institute of Health U24 DK092993 |
