Summary of Study ST000257
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000209. The data can be accessed directly via it's Project DOI: 10.21228/M8CP4Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000257 |
| Study Title | NIH WCMC Pilot & Feasibility Project: “Metabolite changes associated with weight loss” |
| Study Type | Feeding study |
| Study Summary | Targeted metabolomic analyses of oxylipins, endocannabinoids, and ceramides were performed on 18 mouse liver, adipose, hypothalamus, plasma, and muscle samples collected from mice euthanized at 18 weeks following consumption of three diet regimens that induced lean, obese, and weight loss phenotypes. Samples were analyzed by UPLC-MS/MS using a Waters Acquity UPLC and detected on an API 4000 QTrap (AB Sciex, Framingham, MA, USA) by multiple reaction monitoring (MRM) after negative mode electrospray ionization. |
| Institute | University of California, Davis |
| Department | Nutrition |
| Laboratory | Newman Lab |
| Last Name | Newman |
| First Name | John |
| Address | 430 W. Health Sciences Dr., Davis, CA 95616 |
| john.newman@ars.usda.gov | |
| Phone | +1-530-752-1009 |
| Submit Date | 2015-01-16 |
| Num Groups | 3 |
| Total Subjects | 18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Uploaded File Size | 128 MB |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-09-24 |
| Release Version | 1 |
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Project:
| Project ID: | PR000209 |
| Project DOI: | doi: 10.21228/M8CP4Q |
| Project Title: | Metabolite changes associated with weight loss |
| Project Type: | Feeding study |
| Project Summary: | This study aims to identify changes in metabolites that occur in metabolic tissues, including liver, adipose (epididymal), hypothalamus, and muscle, as well as plsma, in the obese state that are long-lasting and not reversed by weight loss. Mice were divided into three groups termed LFD (low fat diet), HFD (high fat diet), and SW (switch diet from HFD to LFD)(n = 6 per group). All mice were fed normal chow until 12 weeks of age. The LFD group were fed a low fat diet (10% fat) ad libitum for 18 weeks. The HFD diet group were fed high fat diet (60% fat) ad libitum for 18 weeks. The SW group were fed high fat diet for 9 weeks, then low fat diet for 9 weeks after which their body weights returned to similar levels as the LFD group. Each group was analyzed for metabolite and gene expression changes. |
| Institute: | University of California, San Diego |
| Department: | Department of Medicine |
| Laboratory: | Osborn |
| Last Name: | Osborn |
| First Name: | Olivia |
| Address: | 9500 Gilman Dr., La Jolla, CA 92093 |
| Email: | oosborn@ucsd.edu |
| Phone: | +1-858-822-6645 |
| Funding Source: | National Institute of Health U24 DK092993 |
