Summary of Study ST000353
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000282. The data can be accessed directly via it's Project DOI: 10.21228/M8G308 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000353 |
| Study Title | The Development of Metabolomic Markers in African Bermudagrass (C. transvaalensis) for Sting Nematode (Belonolaimus longicaudatus) Response |
| Study Type | Disease response in terms of nematode reproduction and root weight |
| Study Summary | The objective of the proposed pilot study is to identify metabolites up- and down-regulated in African bermudagrass that are tolerant and sensitive to the sting nematode and develop metabolomic markers for the highest expressed metabolites associated with tolerance. Future work will include additional accessions and species of bermudagrass, and testing under field conditions. Bermudagrass accessions identified as tolerant or sensitive by Pang et al. (2011) will be assessed under controlled greenhouse conditions to identify metabolites linked to sting nematode tolerance. Nematode response will be quantified through determination of root length and weight and the number of nematodes present 136 days after inoculation. Higher root length and weight indicate tolerance or resistance. Higher nematode counts indicate greater reproduction (i.e. a susceptible plant), while lower counts indicate that the accession may have some resistance. Metabolites from root tissue of these accessions will be compared to identify those associated with tolerance/resistance, and those that are associated with nematode infestation by comparing inoculated plants to uninoculated controls. Metabolomic markers will then be developed for the metabolites associated with tolerance/resistance. These markers will be used to guide future screening of bermudagrass accessions for breeding nematode-tolerant or -resistant varieties. |
| Institute | University of Florida |
| Department | SECIM |
| Laboratory | Turfgrass Breeding |
| Last Name | Benda |
| First Name | Nicole |
| Address | 2005 SW 23rd St |
| nbenda@ufl.edu | |
| Phone | 352-792-4561 |
| Submit Date | 2015-11-13 |
| Num Groups | 3 |
| Total Subjects | 42 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-07-10 |
| Release Version | 1 |
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Project:
| Project ID: | PR000282 |
| Project DOI: | doi: 10.21228/M8G308 |
| Project Title: | The Development of Metabolomic Markers in Bermudagrass (Cynodon spp.) for Sting Nematode (Belonolaimus longicaudatus) Response |
| Project Summary: | The majority of golf courses in Florida experience damage from plant-parasitic nematodes and more than 80% of golf courses in Florida are at risk for nematode-related damage (Crow, 2005). With limited availability of nematicides and lack of tolerant cultivars, turf managers need new genetically resistant cultivars. Use of resistant or tolerant grasses is the most efficient, environmentally friendly and, in the long run, least costly component of IPM. Current screening of new genotypes for nematode resistance is labor- and time-intensive, with three months required soley for nematode development on test plants. We intend to develop metabolomic markers or a signature pattern associated with nematode resistance or tolerance. Screening plants for these markers or a signature pattern of would greatly speed up the screening process and development of resistant cultivars. |
| Institute: | University of Florida |
| Department: | Agronomy |
| Laboratory: | Turfgrass Breeding |
| Last Name: | Kenworthy |
| First Name: | Kevin |
| Address: | 2005 SW 23rd Street, Gainesville FL 32611 |
| Email: | nbenda@ufl.edu |
| Phone: | 352-792-4561 |
