Summary of Study ST001287
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000869. The data can be accessed directly via it's Project DOI: 10.21228/M8C40P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001287 |
| Study Title | Luminal succinate in UC-HMA (human microbiota-associated) mice. |
| Study Type | MS analysis |
| Study Summary | Patients with ulcerative colitis (UC) are known to be at higher risk for Clostridium difficile (C. difficile) infection (CDI), and CDI in UC patients is recognized as a major clinical problem because it worsens UC outcome. In order to assess the role of gut dysbiosis, seen in UC patients, we have established a human microbiota-associated mouse model in which germ-free mice are colonized with gut microbiota from UC patients. Utilizing this model, we found that UC microbiota colonized HMA mice (UC-HMA mice) were susceptible to CDI. To address the mechanism by which UC-HMA mice are unable to acquire the colonization resistance against C. difficile, we analyzed the luminal metabolites in UC-HMA mice, especially in succinate which is a crucial metabolite doe the growth of C. difficile. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | Ann Arbor, MI |
| mkachman@med.umich.edu | |
| Phone | 734-232-0842 |
| Submit Date | 2019-12-11 |
| Num Groups | 63 |
| Total Subjects | 3 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-01-13 |
| Release Version | 1 |
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Project:
| Project ID: | PR000869 |
| Project DOI: | doi: 10.21228/M8C40P |
| Project Title: | Association of luminal succinate in UC-Mb mice and succinate consuming bacteria |
| Project Type: | MS analysis |
| Project Summary: | Measure the levels of luminal succinate in UC-Mb mice (UC-Mb colonized human gnotobiotic mise) and succinate consuming bacteria inoculated UC-Mb mice to elucidate the association between the increased abundance of succinate consuming bacteira and decreased amounts of luminal succinate in CDI-susceptible UC-Mb mice. |
| Institute: | University of Michigan |
| Department: | Internal Medicine-Gastroenterology |
| Laboratory: | Kamada Lab |
| Last Name: | Kamada |
| First Name: | Nobuhiko |
| Address: | Ann Arbor, MI |
| Email: | nkamada@umich.edu |
| Phone: | 734-763-2142 |
