Summary of Study ST001654
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001060. The data can be accessed directly via it's Project DOI: 10.21228/M8PH55 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001654 |
| Study Title | Metabolic profiling of plasma collected from Peromyscus leucopus and Mus musculus following LPS treatment |
| Study Type | Untargeted Metabolomic profiling |
| Study Summary | Small molecule metabolites were extracted from plasma from Peromyscus leucopus and Mus musculus BALB/c and analyzed by liquid chromatography couple with mass spectrometry (LC-MS). XCMS software was used for peak picking, grouping and retention time correction from LC-MS data and generated a molecular feature spreadsheet. Pathway way enrichment analysis was carried out using Mummichog pathway analysis software using data from feature table. |
| Institute | Colorado State University |
| Department | Microbiology, Immunology and Pathology |
| Laboratory | Belisle Lab |
| Last Name | Nurul |
| First Name | Islam |
| Address | 3185 Rampart Road, Fort Collins, CO 80521 |
| nurul.islam@colostate.edu | |
| Phone | 970 492 4249 |
| Submit Date | 2021-01-13 |
| Num Groups | 4 |
| Total Subjects | 40 |
| Num Males | 20 |
| Num Females | 20 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-03-15 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001060 |
| Project DOI: | doi: 10.21228/M8PH55 |
| Project Title: | Investigation of infection-tolerance phenomenon in mammalian reservoir of Lyme disease, Peromyscus leucopus |
| Project Type: | Comparative study of Peromyscus leucopus and Mus musculus, BALB/c, metabolic response to endotoxin treatment |
| Project Summary: | Infection-tolerance mechanism were investigated in a natural reservoir of Lyme disease, Peromyscus leucopus, white-footed deermice following exposure of a single dose of lipopolysaccharide endotoxin. The multiparametric responses of this treatment were compared to widely used mouse model, Mus musculus BALB/c. Following LPS and saline treatment, samples from blood, spleen and liver tissues were collected postmortem from both animals and subjected to RNA-seq, untargeted metabolomics and specific RT-qPCR. |
| Institute: | Colorado State University |
| Department: | Microbiology, Immunology and Pathology |
| Laboratory: | Belisle Lab |
| Last Name: | Nurul |
| First Name: | Islam |
| Address: | 3185 Rampart Road, Fort Collins, CO 80521 |
| Email: | nurul.islam@colostate.edu |
| Phone: | 970 492 4249 |
| Funding Source: | NIH and the Office of the Assistant Secretary of Defense for Health Affairs through the Tick Borne Disease Research Program |
| Project Comments: | N/A |
| Publications: | N/A |
| Contributors: | Gabriela Balderrama Gutierrez, Ana Milovic, Vanessa J. Cook, M. Nurul Islam, Youwen Zhang, Hippokratis Kiaris, John T. Belisle, Ali Mortazavi and Alan G. Barbour. |
