Summary of Study ST002055

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001300. The data can be accessed directly via it's Project DOI: 10.21228/M8P69K This work is supported by NIH grant, U2C- DK119886.

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Study IDST002055
Study TitleMetabolomic Profiling of Human Pluripotent Stem Cell Differentiation into Lung Progenitors
Study SummaryMetabolism is vital to cellular function and tissue homeostasis during human lung development. In utero, embryonic pluripotent stem cells undergo endodermal differentiation towards a lung progenitor cell fate that can be mimicked in vitro using induced human pluripotent stem cells (hiPSCs) to study genetic mutations. To identify differences between wild type and surfactant protein B (SFTPB)-deficient cell lines during endoderm specification towards lung, we used an untargeted metabolomics approach to evaluate the developmental changes in metabolites. We found that the metabolites most enriched during the differentiation from pluripotent stem cell to lung progenitor cell, regardless of cell line, were sphingomyelins and phosphatidylcholines, two important lipid classes in fetal lung development. The SFTPB mutation had no metabolic impact on early endodermal lung development. The identified metabolite signatures during lung progenitor cell differentiation may be utilized as biomarkers for normal embryonic lung development.
Institute
The Hospital for Sick Children
Last NamePost
First NameMartin
Address555 University Avenue
Emailmartin.post@sickkids.ca
Phone4168136772
Submit Date2021-06-29
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2022-01-13
Release Version1
Martin Post Martin Post
https://dx.doi.org/10.21228/M8P69K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001300
Project DOI:doi: 10.21228/M8P69K
Project Title:Metabolomic Profiling of Human Pluripotent Stem Cell Differentiation into Lung Progenitors
Project Type:Developmental metabolites and Biomarker discovery
Project Summary:Metabolism is vital to cellular function and tissue homeostasis during human lung development. In utero, embryonic pluripotent stem cells undergo endodermal differentiation towards a lung progenitor cell fate that can be mimicked in vitro using induced human pluripotent stem cells (hiPSCs) to study genetic mutations. To identify differences between wild type and surfactant protein B (SFTPB)-deficient cell lines during endoderm specification towards lung, we used an untargeted metabolomics approach to evaluate the developmental changes in metabolites. We found that the metabolites most enriched during the differentiation from pluripotent stem cell to lung progenitor cell, regardless of cell line, were sphingomyelins and phosphatidylcholines, two important lipid classes in fetal lung development. The SFTPB mutation had no metabolic impact on early endodermal lung development. The identified metabolite signatures during lung progenitor cell differentiation may be utilized as biomarkers for normal embryonic lung development.
Institute:The Hospital for Sick Children
Last Name:Post
First Name:Martin
Address:555 University Avenue
Email:martin.post@sickkids.ca
Phone:4168136772
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