Summary of Study ST000020
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000019. The data can be accessed directly via it's Project DOI: 10.21228/M8TG6F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000020 |
| Study Title | Biomarker Discovery in Knee Osteoarthritis (I) |
| Study Type | Biomarker Discovery in Knee Osteoarthritis |
| Study Summary | The goal of the study was to determine whether there is a set of metabolites that differentiate people who have knee OA and show radiographic disease progression over 18 months from those who have knee OA and do not show disease progression over the same time period. |
| Institute | University of North Carolina |
| Department | Systems and Translational Sciences |
| Laboratory | Sumner Lab |
| Last Name | Sumner |
| First Name | Susan |
| Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
| susan_sumner @unc.edu | |
| Phone | 704-250-5066 |
| Submit Date | 2014-02-28 |
| Num Groups | 4 |
| Total Subjects | 88 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Uploaded File Size | 53 M |
| Analysis Type Detail | NMR |
| Release Date | 2018-08-27 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP000033 |
| Sampleprep Summary: | Chenomx Internal Standard solution (70 ul) and 230 ul D20 was added to each of the 88 urine sample (400 ul), vortexed for 30s, and centrifuged at 12000 rcf for 5min. Chenomx ISTD (Chenomx, Edmonton, Alberta, Canada) contains 5mM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS, Chemical Shift Indicator), 100 mM Imidazole (pH indicator), and 0.2% NaN3 (to inhibit bacterial growth) in D2O. 600 µl aliquot of the supernatant was transferred into 5mm NMR tubes (Bruker-Biospin, Germany). Phenotypic pooled urine samples were made by combining 200 µl aliquots from each of the study samples belonging to the same phenotype (Progressors-Baseline, Progressors-follow up, Non-progressors-baseline, and Non-progressors-follow up). In addition, a combined phenotypic pooled sample was also prepared by using 500 µl aliquot from each of the phenotypic pooled sample. Pooled NMR samples were prepared as described above and used as quality check (QC) samples. |
| Sampleprep Protocol ID: | RTI NMR SERUM CHENOMX method |
| Sampleprep Protocol Filename: | RTI_WOA_IDEA_Metabolomics_Procedure_Feb28_2014.docx |
| Processing Method: | Dilution using a mixture of D2O and Chenomx ISTD |
| Processing Storage Conditions: | On ice |
| Extraction Method: | None |
