Summary of Study ST000121
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000108. The data can be accessed directly via it's Project DOI: 10.21228/M8GW2Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000121 |
| Study Title | Impact of anesthesia and euthanasia on metabolomics of mammalian tissues: studies in a C57BL/6J mouse model |
| Study Type | Anesthesia effect study |
| Study Summary | We examined the effect of several commonly-used methods of anesthesia and euthanasia for collection of skeletal muscle, liver, heart, adipose and serum of C57BL/6J mice. The data revealed tissue-specific impacts of different anesthesia and euthanasia strategies. Based on these findings, we present a more optimal collection strategy mammalian tissues and recommend that rodent tissues intended for metabolomics studies be collected under anesthesia rather than post-euthanasia. |
| Institute | University of Michigan |
| Department | Internal Medicine |
| Laboratory | Evans Lab / Burant Lab |
| Last Name | Evans |
| First Name | Charles |
| Address | 6321 Brehm Tower, 1000 Wall Street, Ann Arbor MI 48105 |
| chevans@umich.edu | |
| Phone | 734-232-8177 |
| Submit Date | 2014-11-21 |
| Num Groups | 6 |
| Total Subjects | 49 |
| Raw Data Available | No |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-02-01 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP000137 |
| Sampleprep Summary: | Frozen tissue samples were pulverized in a liquid nitrogen-chilled mortar and pestle, then sonicated in cold extraction solvent (7:2:1 methanol:chloroform:water) at an approximate ratio of 30 mg tissue (wet mass) /1 mL of solvent. After allowing samples to rest on ice 5 minutes, they were centrifuged to pellet precipitated protein and cell debris; the supernatant was placed in autosampler vials for direct LC-MS analysis. For serum, extraction solvent (1:1:1 methanol:acetonitrile:acetone) was added to serum at a 4:1 solvent:serum ratio, vortexed to mix, then centrifuged and supernatant recovered for LC-MS analysis. |
| Sampleprep Protocol Filename: | PROTOCOLS_mouse_anesthesia.pdf |
| Processing Method: | Cryo-pulverization + sonication in extraction solvent |
