Summary of Study ST000140
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000125. The data can be accessed directly via it's Project DOI: 10.21228/M8DW2B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000140 |
| Study Title | Cell Rinsing Solution Comparison |
| Study Type | Cell Rinsing Solution Comparison |
| Study Summary | Aliquots of Jurkat T-lymphocyte cells were washed with 5 different rinsing solutions (0.3% amm. formate, 0.3% amm. acetate, 0.9% NaCl, 1 M PBS, 100 mM PBS) and ran in triplicate to monitor the effect of ion suppression on the electrospray ionization signal. |
| Institute | University of Florida |
| Department | Dept. of Chemistry/SECIM |
| Laboratory | Biomedical Mass Spectrometry Lab |
| Last Name | Ulmer |
| First Name | Candice |
| Address | P.O. Box 117200, Gainesville, FL 32611 |
| czulmer@chem.ufl.edu | |
| Phone | (352) 392-0515 |
| Submit Date | 2015-03-02 |
| Num Groups | 5 |
| Total Subjects | 15 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Uploaded File Size | 3.1 G |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-03-03 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP000157 |
| Sampleprep Summary: | 80% MeOH metabolite extraction |
| Sampleprep Protocol ID: | SOP_PP_02 |
| Sampleprep Protocol Filename: | SOP_PP_02_CZU.pdf |
| Processing Method: | cell lysis |
| Processing Storage Conditions: | on ice |
| Extraction Method: | 80% MeOH |
| Extract Concentration Dilution: | 1e6 cells/ 2 mL of extraction solvent |
| Sample Resuspension: | 30 μL 0.1% formic acid in water |
| Sample Spiking: | internal standards: (1) L-tryptophan-2,3,3-d3; (2) creatine-d3; (3) d-leucine-d10; (4) caffeine-d3 |
| Cell Type: | Jurkat, Clone E6-1 |
