Summary of Study ST000255
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000207. The data can be accessed directly via it's Project DOI: 10.21228/M8N30T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000255 |
Study Title | NIH WCMC Pilot & Feasibility Project: Metabolomics of Neonatal Pulmonary Hypertension in human |
Study Type | Case-control study |
Study Summary | Targeted metabolomic analyses of oxylipins were performed on 40 human umbilical cord plasma samples collected from infants born prematurely (gestational age <37 weeks). Samples were analyzed by UPLC-MS/MS using a Waters Acquity UPLC and detected on an API 4000 QTrap (AB Sciex, Framingham, MA, USA) by multiple reaction monitoring (MRM) after negative mode electrospray ionization. |
Institute | University of California, Davis |
Department | Nutrition |
Laboratory | Newman Lab |
Last Name | Newman |
First Name | John |
Address | 430 W. Health Sciences Dr., Davis, CA 95616 |
john.newman@ars.usda.gov | |
Phone | +1-530-752-1009 |
Submit Date | 2015-09-03 |
Num Groups | 4 |
Total Subjects | 16 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Uploaded File Size | 32 M |
Analysis Type Detail | LC-MS |
Release Date | 2015-09-14 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000281 |
Sampleprep Summary: | Plasma was added directly to a Waters Ostro plate, mixed with the surrogates, antioxidants, and acetonitrile and the extract eluted through. The extracts were dried, reconstituted in 100?L 50:50 methanol:acetonitrile with internal standards 1?phenyl?3?hexanoic acid urea and 1?cyclohexyl?3-dodecanoic acid urea and filtered at 0.1 µm prior to analysis. |
Sampleprep Protocol Filename: | NewmanLab-FreeOxys-Protocol-P.pdf |
Extraction Method: | Ostro plate |
Extract Cleanup: | Ostro plate |
Extract Storage: | - 20 °C |
Sample Resuspension: | 100 µL |
Sample Spiking: | See sample prep protocol file |
Organ: | Umbilical cord plasma |