Summary of Study ST000469

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000360. The data can be accessed directly via it's Project DOI: 10.21228/M8HK59 This work is supported by NIH grant, U2C- DK119886.


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Study IDST000469
Study TitleEnterococcus faecalis nucleotide profiles - pilot
Study TypeComparison between wild-type and mutant strain, as well as providing varying cell count input for WT
Study SummaryE. faecalis OG1RF and rel/relQ will be grown in FMC-AUG to OD600 = 0.25. 3E10 cells will be harvested by fast filtration, scraped off of membrane into ice-cold PBS, and pelleted by centrifugation. This process will be repeated with OG1RF only to harvest 3E9 and 3E8 cells. A second OG1RF sample of 3E10 cells will be scraped into formic acid; only acid-soluble extract willl be sent for this sample.
University of Florida
Last NameKajfasz
First NameJessica
Address1395 Center Drive, PO Box 100424 Gainesville, FL 32610
Submit Date2016-09-10
Num Groups5
Total Subjects5
Raw Data AvailableYes
Raw Data File Type(s)raw
Analysis Type DetailLC-MS
Release Date2017-10-03
Release Version1
Jessica Kajfasz Jessica Kajfasz application/zip

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Sample Preparation:

Sampleprep ID:SP000497
Sampleprep Summary:NAD|NADH
Sampleprep Protocol Filename:SB_NAD-ATP_Assay_Agilent.pdf