Summary of Study ST000497

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000374. The data can be accessed directly via it's Project DOI: 10.21228/M8Q60G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST000497
Study Title	M-CMV infected Nicotiana tabacum
Study Type	LC-MS & GC-MS
Study Summary	M-CMV-infected N. tabacum plants with six symptoms (vein clearing, mosaic, chlorosis, partial green recovery, complete green recovery and secondary mosaic) were analyzed by LC-MS & GC-MS. In addition, the pathogenesis biomarker might be found by this untargeted global metabolomic analysis.
Institute	Chinese Academy of Inspection and Quarantine
Department	Institute of Plant Quarantine of China
Last Name	Lei
First Name	Rong
Address	Rong Hua Nan Lu 11, Yi Zhuang, Beijing, China
Email	leirong2009@gmail.com
Phone	86-10-53897620
Submit Date	2016-10-23
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	GC-MS/LC-MS
Release Date	2017-11-20
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000525
Sampleprep Summary:	Samples were prepared using the automated MicroLab STAR® system (Hamilton Company, Salt Lake City, UT). A 6-mg quantity of each lyophilized leaf sample was thawed on ice and extracted using the automated MicroLab STAR system in 400 L of methanol (LC-MS Ultra CHROMASOLV®, Riedel-de Haёn) containing recovery standards. The samples were prepared using a proprietary series of organic and aqueous extractions to remove the protein fraction while maximizing the recovery of small molecules. The resulting extract was divided into two fractions; one for analysis by liquid chromatography (LC) and one for analysis by gas chromatography (GC). The samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. Each sample was then frozen and dried under vacuum, followed by preparation for either LC/MS or GC/MS.

Sampleprep ID:

SP000525

Sampleprep Summary:

Samples were prepared using the automated MicroLab STAR® system (Hamilton Company, Salt Lake City, UT). A 6-mg quantity of each lyophilized leaf sample was thawed on ice and extracted using the automated MicroLab STAR system in 400 L of methanol (LC-MS Ultra CHROMASOLV®, Riedel-de Haёn) containing recovery standards. The samples were prepared using a proprietary series of organic and aqueous extractions to remove the protein fraction while maximizing the recovery of small molecules. The resulting extract was divided into two fractions; one for analysis by liquid chromatography (LC) and one for analysis by gas chromatography (GC). The samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. Each sample was then frozen and dried under vacuum, followed by preparation for either LC/MS or GC/MS.