Summary of Study ST000550

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000403. The data can be accessed directly via it's Project DOI: 10.21228/M8ZG6P This work is supported by NIH grant, U2C- DK119886.

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Study IDST000550
Study TitleMetabolomics marker of brown adipose tissue in men
Study SummaryObjective: We aimed to identify metabolites in serum that are associated with BAT volume and activity in men. Methods: We assessed 163 metabolites in fasted serum of a cohort of twenty two healthy lean men (age 24.1 (21.7 – 26.6) years, BMI 22.1 (20.5 – 23.4) kg/m2) who subsequently underwent a cold-induced [18F]FDG PET-CT scan to assess BAT volume and activity. In addition, we included three replication cohorts consisting of in total thirty-seven healthy lean men that were similar with respect to age and BMI compared to the discovery cohort.
Institute
Leiden University Medical Center
Last NameMook-Kanamori
First NameDennis
AddressPO Box 9600, 2300 RC, Leiden, the Netherlands
Emaild.o.mook@lumc.nl
Phone+31715265623
Submit Date2017-01-31
Num Groups5
Total Subjects59
Num Males59
Analysis Type DetailLC-MS
Release Date2017-07-10
Release Version1
Dennis Mook-Kanamori Dennis Mook-Kanamori
https://dx.doi.org/10.21228/M8ZG6P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP000579
Sampleprep Summary:Targeted metabolomics Metabolomic measurements were performed on serum samples taken at thermoneutral and cold conditions at the Genome Analysis Center at the Helmholtz Zentrum, Munich, Germany, using the Biocrates AbsoluteIDQTM p150 kit (BIOCRATES Life Science AG, Innsbruck, Austria) and ESI-FIA-MS/MS measurements (Menni et al. 2013). The assay allows simultaneous quantification of 163 small molecule metabolites within 10 µL serum. The assay includes free carnitine, 40 acylcarnitines, 14 amino acids (13 proteinogenic + ornithine), hexoses (of which approx. 90-95% is glucose), 92 glycerophospholipids including 15 lysophosphatidylcholines (LysoPCs) and 77 phosphatidylcholines (PCs), and 15 sphingolipids (Menni et al. 2013; Illig et al. 2010). Quantification of the metabolites was achieved by reference to appropriate internal standards. The assay has been previously described (Menni et al. 2013; Illig et al. 2010).
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