Summary of Study ST000600

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000437. The data can be accessed directly via it's Project DOI: 10.21228/M8K31K This work is supported by NIH grant, U2C- DK119886.


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Study IDST000600
Study TitleNEFA Profile Response to Triphenyl Phosphate Exposure
Study SummaryThis study aims to identify changes in non-esterified fatty acid (NEFAs) in the plasma with triphenyl phosphate (TPP) exposure. UC Davis type 2 diabetes mellitus (UCD-T2DM) rats were treated with TPP or not treated. Each group was analyzed for non-esterified fatty acid (NEFA) changes to investigate alterations in NEFAs due to TPP exposure. Targeted analysis of NEFA in rat plasma samples was performed by the Newman lab.
University of California, Davis
DepartmentUSDA Western Human Nutrition Research Center
Last NameNewman
First NameJohn
Address430 W. Health Sciences Dr., Davis, CA 95616
Submit Date2017-04-27
Study CommentsThe samples included a high degree of hemolysis exhibited in the plasma. One sample was lost during processing (Group E- Subject 78). Two samples were outliers for multiple analytes and were not included in the final data (E-117 & T-28). Of the samples reported in this data set, there were no missing values.
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC-MS
Release Date2017-07-10
Release Version1
John Newman John Newman application/zip

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Sample Preparation:

Sampleprep ID:SP000630
Sampleprep Summary:Plasma non-esterified fatty acids (NEFAs) were isolated as previously described by Smedes (1). and Gladine et al. (2). Specifically, plasma aliquots (100 mL) were enriched with 5 mL 0.2 mg/ml butylated hydroxytoluene/EDTA in 1:1 methanol:- water, and a suite of extraction surrogates, which included deuterated-tri-palmitoyl glycerol (d31-16:0-TG; CDN Isotopes, Pointe-Claire, Quebec, Canada), deuterated distearoylphosphotidylcholine (d35-18:0-PC; Avanti Polar Lipids, Alabaster, Alabama), dodeca-(9E)-enoyl cholesterylesters (22:1n9-CE; NuChek Prep, Elysian MN) and dodecatrienoic acid (22:3n3; NuChek Prep). Lipids were then extracted with 10:8:11 cylcohexane: 2- propanol:ammonium acetate. Briefly, enriched samples were mixed with cyclopropane/2-propanol, phases were split with ammonium acetate, the organic phase was isolated and the aqueous phase was re-extracted with cyclohexane. The combined organic total lipid extract was reduced to dryness and reconstituted in 200 µL of 1:1 methanol/toluene and the total lipid extract was used to quantify plasma fatty acids as methyl esters by gas chromatography-mass spectrometry (GC-MS). It was derivitized by adding 45 μL 2M (trimethylsilyl) diazomethane in hexanes and spiked with 15:1n5 free acid to track methylation efficiency. Next, it was brought to a final volume of 200 mL with 90:10 methanol/toluene (v/v) and left at room temperature for 30 min, before being brought to dryness. The remaining fatty acid methyl esters (FAMEs) were re-constituted in 300 mL Hexane plus 10 uL of 44 mM tricosanoate methyl ester (23:0; NuChek Prep), vortexed, and 100 uL was transferred to a GC-MS Vial for analysis.
Sampleprep Protocol Filename:NEFA_Plasma_Newman_Data_Report.docx