Summary of Study ST000671

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000474. The data can be accessed directly via it's Project DOI: 10.21228/M8SG7H This work is supported by NIH grant, U2C- DK119886.

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Study IDST000671
Study TitleLCR/HCR rat mitochondrial study
Study TypeMS analysis
Study SummaryLCR and HCR rats (from Nathan Qi's 2010 study) were dissected at rest or following 10 minutes of exercise. Mitochondria were isolated from frozen gastrocnemius skeletal muscle. Samples consist of these isolated mitochondria suspended in 50 uL of mitochondrial isolation buffer (IBM containing sucrose, salts, etc; see Katie Overmyer's notebook E, p~23).
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-05
Num Groups4
Total Subjects12
Study CommentsRecommend extracting entire sample suspension, NOT pelleting and removing supernatant. Quantities are likely to be small (~1-3mg tissue each?) Protein assays were performed to estimate quantities; see Katie's notebook or contact Charles for these protein concentration estimates, but also RECOMMEND SAVING RESIDUAL PELLET to allow further normalization.
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8SG7H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP000701
Sampleprep Summary:-
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf
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