Summary of Study ST000982
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000671. The data can be accessed directly via it's Project DOI: 10.21228/M8XX0K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000982 |
Study Title | Analysis the concentration of betaine, choline, TMA, TMAO and amino acids in plasma in Megalobrama amblycephala. |
Study Summary | Analysis the concentration of betaine, choline, TMA, TMAO and amino acids in plasma in Megalobrama amblycephala by HPLC-MS/MS. |
Institute | Huazhong Agricultural University |
Last Name | Xu |
First Name | Jia |
Address | Lion rock street, hung shan district, Wuhan, HuBei, 430070, China |
xujia2018hzau@163.com | |
Phone | 13018097215 |
Submit Date | 2018-06-25 |
Analysis Type Detail | LC-MS |
Release Date | 2018-08-27 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001028 |
Sampleprep Summary: | Fifty μl of protein precipitant (containing NVL) was added to 50 μl of serum solution of each sample, mixed thoroughly, and centrifuged at 13200 rpm for 4 min. The supernatant (10 μl) was mixed with 50 μl methanol borate buffer (0.1 M, pH=8.8) thoroughly. For amino acids, the above solution was mixed with 20 μl of diethyl ethoxymethylene-malonate at 55°C for 15 min. |