Summary of Study ST001032

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000690. The data can be accessed directly via it's Project DOI: 10.21228/M8GQ3M This work is supported by NIH grant, U2C- DK119886.


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Study IDST001032
Study TitleSingle-cell Profiling of Cationic and Anionic Metabolites in Live Frog (Xenopus) Embryos using Microprobe Capillary Electrophoresis Mass Spectrometry
Study TypeMetabolic profiling of single cells
Study SummaryThe goal of this study was to enable the analysis of anionic and cationic metabolites from the same identified single cell in Xenopus laevis embryos. A 10 nL portion of identified animal-ventral (V1) cells was aspirated from 8-cell embryos, and metabolites were extracted from the aspirate, before characterization of cationic and anionic compounds using a custom-built capillary electrophoresis (CE) electrospray ionization (ESI) mass spectrometry platform. A total of ~250 cationic molecular features and ~150 anionic molecular features were detected, including 76 metabolites that were identified in this study. Pathway analysis of the identified metabolites highlighted arginine-proline metabolism of significance.
University of Maryland
DepartmentDepartment of Chemistry & Biochemistry
LaboratoryNemes Laboratory
Last NameNemes
First NamePeter
Address0107 Chemistry Building 8051 Regents Drive
Submit Date2018-08-08
Num Groups4 biological replicates (each different cell from a different embryo) + 1-to-2 technical replicates (same extract analyzed multiple times)
Total Subjects4 different V1 cells were analyzed, each from a different embryo
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2019-09-23
Release Version1
Peter Nemes Peter Nemes application/zip

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Sample Preparation:

Sampleprep ID:SP001078
Sampleprep Summary:Ca. 10 nL of cell content were aspirated from identified cells. The aspirated material was ejected into ~4 uL of aqueous mixture of 40% acetonitrile and 40% methanol to extract metabolites.
Sampleprep Protocol Filename:nemes_20180725_143242_PR_SP_Liu_2018_Metabolomics_Workbench_Protocol.pdf
Processing Method:On ice, then extracts stored at -80 degC until analysis.
Processing Storage Conditions:On ice
Extraction Method:In cold aqueous mixture of 40% acetonitrile and 40% methanol.
Extract Enrichment:none
Extract Cleanup:none
Extract Storage:-80℃
Subcellular Location:Unknown