Summary of Study ST001149

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000768. The data can be accessed directly via it's Project DOI: 10.21228/M8DH49 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001149
Study TitlePlasmodium Niemann-Pick Type C1-Related Protein is a Druggable Target Required for Parasite Membrane Homeostasis
Study TypeDrug Exposure
Study SummaryMetabolic examination/confirmation of resistance mutations and GFP tagging observed in study
Institute
Pennsylvania State University
DepartmentBMB
LaboratoryLlinás Laboratory
Last NameLlinás
First NameManuel
AddressW-122 Millennium Science Complex, Pollock Road
Emaileso5005@psu.edu
Phone8148673527
Submit Date2019-02-15
Raw Data AvailableYes
Analysis Type DetailLC-MS
Release Date2019-05-15
Release Version1
Manuel Llinás Manuel Llinás
https://dx.doi.org/10.21228/M8DH49
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP001222
Sampleprep Summary:Samples washed in PBS, spun down, aspirated, extracted in 90% methanol with labelled aspartate, then dried, resuspended in water labelled with chlorpropamide
Sampleprep Protocol Filename:Metabolite_Extraction_for_LCMS_2017_Llinas.pdf
Processing Method:Wash, Lysis, Drying, resuspension
Processing Storage Conditions:On ice
Extraction Method:90% Methanol Lysis
Extract Storage:-80℃
Sample Resuspension:100 uL water with chlorpropamide
Sample Spiking:Labelled Aspartate, Chlorpropamide
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