Summary of Study ST001200

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000808. The data can be accessed directly via it's Project DOI: 10.21228/M87M3D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001200
Study TitleBiological Responses to Tobacco Smoke Exposure in III Children: Inflammatory Processes and the Oral Microbiome
Study SummaryThis project evaluates the biological response to overall tobacco smoke (OTS) exposure among pediatric emergency patients enrolled in a randomized-controlled intervention trial aimed at reducing secondhand smoke exposure. The effects of OTS as measured by salivary continine on salivary metabolic profiles are measured by untargeted high resolution metabolomics, comparing higher and lower levels of OTS.
Institute
Emory University
DepartmentSchool of Medicine
LaboratoryClincal Biomarkers Laboratory
Last NameUppal
First NameKaran
AddressNA
Emailkuppal2@emory.edu
Phone(404) 727 5027
Submit Date2019-06-12
Total Subjects284
Study CommentsBoth CHEAR pooled saliva samples and Clinical Biomarker Laboratory pooled plasma samples were used
Raw Data AvailableYes
Raw Data File Type(s)mzXML, raw(Thermo)
Chear Study2017-1762
Analysis Type DetailLC-MS
Release Date2020-05-11
Release Version1
Karan Uppal Karan Uppal
https://dx.doi.org/10.21228/M87M3D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:SP001275
Sampleprep Summary:Samples were prepared for metabolomics analysis using established methods(Johnson et al. (2010). Analyst; Go et al. (2015). Tox Sci). Prior to analysis, plasma aliquots were removed from storage at -80 degrees C and thawed on ice. Each cryotube was then vortexed briefly to ensure homogeneity, and 50 microliters was transferred to a clean microfuge tube. Immediately after, the plasma was treated with 100 microliters of ice-cold LC-MS grade acetonitrile (Sigma Aldrich) containing 2.5 microliters of internal standard solution with eight stable isotopic chemicals selected to cover a range of chemical properties. Following addition of acetonitrile, urine was equilibrated for 30 min on ice, upon which precipitated proteins were removed by centrifuge (14,000 rpm at 4 degrees C for 10 min). The resulting supernatant (100 microliters) was removed, added to a low volume autosampler vial and maintained at 4 degrees C until analysis (<22 h).
Sampleprep Protocol ID:HRM_SP_082016_01
Sampleprep Protocol Filename:EmoryUniversity_HRM_SP_082016_01.pdf
Sampleprep Protocol Comments:Date effective: 30 July 2016
Extraction Method:2:1 acetonitrile: sample followed by vortexing and centrifugation
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