Summary of study ST001260

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000846. The data can be accessed directly via it's Project DOI: 10.21228/M8B97R This work is supported by NIH grant, U2C- DK119886.


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Study IDST001260
Study TitleMetabolic changes of Fusobacterium nucleatum when co-cultured with other oral microbes (part-I)
Study SummaryWe used membrane-separated co-culture systems to globally assess metabolomic changes of Fusobacterium nucleatum when co-cultured with Streptococcus gordonii and/or Veillonella parvula.
Osaka University
DepartmentGraduate School of Dentistry, Department of Preventive Dentistry
Last NameKuboniwa
First NameMasae
AddressYamadaoka 1-8
Submit Date2019-09-26
Analysis Type DetailLC-MS
Release Date2021-04-01
Release Version1
Masae Kuboniwa Masae Kuboniwa application/zip

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Sample Preparation:

Sampleprep ID:SP001336
Sampleprep Summary:To remove protein, 2 ml of chloroform and 0.8 ml of ultrapure water were added to the samples, which were thoroughly mixed and centrifuged at 2300 × g for 5 minutes at 4˚C. The upper aqueous layer was then transferred to ultrafilter tips (Amicon ultrafilter system™) and centrifuged at 9100 × g for 120 minutes at 4˚C. Filtered material was dried under reduced pressure, followed by suspension in 50 µl of ultrapure water.