Summary of Study ST001260

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000846. The data can be accessed directly via it's Project DOI: 10.21228/M8B97R This work is supported by NIH grant, U2C- DK119886.

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Study IDST001260
Study TitleMetabolic changes of Fusobacterium nucleatum when co-cultured with other oral microbes (part-I)
Study SummaryWe used membrane-separated co-culture systems to globally assess metabolomic changes of Fusobacterium nucleatum when co-cultured with Streptococcus gordonii and/or Veillonella parvula.
Institute
Osaka University
DepartmentGraduate School of Dentistry, Department of Preventive Dentistry
Last NameKuboniwa
First NameMasae
AddressYamadaoka 1-8
Emailkuboniwa@dent.osaka-u.ac.jp
Phone81668792922
Submit Date2019-09-26
Analysis Type DetailLC-MS
Release Date2021-04-01
Release Version1
Masae Kuboniwa Masae Kuboniwa
https://dx.doi.org/10.21228/M8B97R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP001336
Sampleprep Summary:To remove protein, 2 ml of chloroform and 0.8 ml of ultrapure water were added to the samples, which were thoroughly mixed and centrifuged at 2300 × g for 5 minutes at 4˚C. The upper aqueous layer was then transferred to ultrafilter tips (Amicon ultrafilter system™) and centrifuged at 9100 × g for 120 minutes at 4˚C. Filtered material was dried under reduced pressure, followed by suspension in 50 µl of ultrapure water.
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